| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Prohibitin 2 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ICC, IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Immunocytochemistry: 0, Western blot: 0 |
| Notes: |
Titration of the Prohibitin 2 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This Prohibitin 2 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q99623 |
| Purity: |
Antigen affinity |
| Description: |
It had no effect on reporter activity in the absence of liganded ER, Montano et al, Mutation analysis showed that an N-terminal domain and a central domain of PHB2 were required for its repressor activity, PHB2 suppressed activation of a reporter gene in a dose-dependent manner, The International Radiation Hybrid Mapping Consortium mapped the gene to chromosome 12, When coexpressed with wildtype ER-alpha or ER-beta(ESR2), also called Repressor of Estrogen Receptor Activity (REA), and it had no effect on the transcriptional activities of other hormone receptors, is a protein that in humans is encoded by the PHB2 gene, (1999) showed that REA enhanced the potency of a dominant-negative ER-alpha mutant and antiestrogens as suppressors of ER-alpha activity in Chinese hamster ovary cells, Prohibitin 2 |
| Immunogen: |
mouse and rat), An amino acid sequence from the middle region of human REA/PHB2 (RPNAQELPSMYQRLGLDY) was used as the immunogen for this Prohibitin 2 antibody (100% homologous in human |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the Prohibitin 2 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Prohibitin 2 |
| Short name: |
Prohibitin 2 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
Prohibitin 2 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
8914 |
| Gene: |
PHBP2 |
More about : PHBP2 |
| Long gene name: |
prohibitin pseudogene 2 |
| Locus: |
11p11, 2 |
| Discovery year: |
1993-11-04 |
| Entrez gene record: |
100418837 |
| Pubmed identfication: |
8244394 |
| Havana BLAST/BLAT: |
OTTHUMG00000166404 |