| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
TFAM |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ICC, IHC-F, IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, 5-1ug/ml, 5-1ug/ml, IHC (Frozen): 0, IHC (Paraffin): 0, Immunocytochemistry: 0, Western blot: 0 |
| Notes: |
Titration of the TFAM antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This TFAM antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q00059 |
| Purity: |
Antigen affinity |
| Description: |
7p, By PCR-based screening of a somatic cell hybrid panel and by fluorescence in situ hybridization, By Southern blot analysis of restriction enzyme digests of human/Chinese hamster somatic cell hybrid lines, It also has a role in mitochondrial DNA replication, Larsson et al, Milatovich et al, Mitochondrial transcription factor A is a key activator of mitochondrial transcription in mammals, Scott(2007) stated that the sequences mapped to chromosomes 7p(TCF6L1) and 11q(MTTF1, This region exhibits syntenic homology with human 10q21, also known as TCF6 or TCF6L2, and 11q, is a 162-amino acid protein that activates transcription of each mitochondrial DNA(mtDNA) strand by binding to an element of approximately 30 nucleotides present in both the light-strand and the heavy-strand promoters, mitochondrial (TFAM), or TCF6L3) are pseudogenes, since transcription generates an RNA primer necessary for initiation of mtDNA replication, to three different chromosomes: chromosomes 10, which they called MTTF1, (1992) mapped TFAM sequences, (1997) mapped the mouse TFAM to the central part of mouse chromosome 10, Transcription factor A |
| Immunogen: |
An amino acid sequence from the C-terminus of human Transcription factor A mitochondrial (RKDLLRRTIKKQRKYGAEEC) was used as the immunogen for this TFAM antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the TFAM antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
TFAM |
| Short name: |
TFAM Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
TFAM (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
11741 |
| Gene: |
TFAM |
More about : TFAM |
| Long gene name: |
mitochondrial , transcription factor A |
| Synonyms gene: |
TCF6 TCF6L2 |
| Locus: |
10q21, 1 |
| Discovery year: |
1991-09-13 |
| GenBank acession: |
BC018628 |
| Entrez gene record: |
7019 |
| Pubmed identfication: |
7789991 |
| RefSeq identity: |
NM_003201 |
| Havana BLAST/BLAT: |
OTTHUMG00000018270 |