| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
HIP HSC70 Interacting Protein |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the HIP antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This HIP antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P50502 |
| Purity: |
Antigen affinity |
| Description: |
HIP stabilized the ADP-bound form of Hsc70, Hohfeld et al, Hohfeld et al, One oligomer bound the ATPase domains of at least two Hsc70 molecules, ST13 is an abundant, They noted that colorectal, Using a yeast 2-hybrid assay, Zhang et al, also known as p48 or HIP, and binding was dependent on activation of the Hsc70 ATPase by Hsp40(DNAJB1), and ovarian carcinomas frequently show loss of heterozygosity at this site, breast, but is absent from the mature receptor complex, highly conserved protein that binds the major cytosolic chaperones heat-shock protein 70-kD (HSP70) and HSP90 during an intermediate stage of steroid receptor assembly, is a protein that in humans is encoded by the ST13 gene, which had a high affinity for a test protein substrate, (1995) concluded that the protein contributes to interactions of HSC70 with target proteins through its own chaperone activity, (1995) showed that rat HIP bound Hsc70(HSPA8), (1998) mapped the gene to chromosome 22q13 by fluorescence in situ hybridization, Suppression of Tumorigenicity 13 |
| Immunogen: |
An amino acid sequence from the N-terminus of human HSC70 Interacting Protein (MDPRKVNELRAFVKMCKQD) was used as the immunogen for this HIP antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the HIP antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
HIP HSC70 Interacting Protein |
| Short name: |
HIP Antibody HSC70 Interacting Protein |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
HIP (Antibody to) HSC70 Interacting Protein |
| Alternative technique: |
antibodies |
| Identity: |
14866 |
| Gene: |
HHIP |
More about : HHIP |
| Long gene name: |
hedgehog interacting protein |
| Synonyms gene name: |
hedgehog-interacting protein |
| Synonyms: |
HIP FLJ20992 |
| Locus: |
4q31, 21 |
| Discovery year: |
2002-02-15 |
| GenBank acession: |
AK024645 |
| Entrez gene record: |
64399 |
| Pubmed identfication: |
11435703 11731473 |
| Havana BLAST/BLAT: |
OTTHUMG00000161428 |