| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
NOXA1 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the NOXA1 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This NOXA1 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q86UR1 |
| Purity: |
Antigen affinity |
| Description: |
Banfi et al, Cells individually transfected with NOX1, Hartz(2007) mapped the gene to chromosome 9q34, Human cell lines or COS-7 cells cotransfected with NOXA1/p51-NOX and p41-NOX and either gp91-PHOX(CYBB) or NOX1 produced superoxide, In vitro binding assays revealed that NOXA1 bound GTP-bound RAC1, It also bound p47-PHOX (NCF1) and p41-NOX (NOXO1), Takeya et al, Using yeast 2-hybrid assays, also called NOX Activator 1 or p51-NOX, and trp436 within the SH3 domain of NOXA1 was required for these interactions, but not GDP-bound RAC1, is an enzyme that in humans is encoded by the NOXA1 gene, or p51-NOX and cells transfected with only p41-NOX and p51-NOX showed no superoxide production, p41-NOX, (2003) mapped the mouse gene to chromosome 2, (2003) showed that the human protein interacted with constitutively active forms of RAC1 and RAC2, 1), 3 based on an alignment of the protein sequence with the genomic sequence(build 36, NADPH oxidase activator 1 |
| Immunogen: |
An amino acid sequence from the middle region of human NADPH oxidase activator 1 (RQVPRGEVFRPHRWHLKHLE) was used as the immunogen for this NOXA1 antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the NOXA1 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
NOXA1 |
| Short name: |
NOXA1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
NADPH oxidase activator 1 (Antibody to) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
Cytoplasm, NOXA1 and IDBG-641039 and ENSBTAG00000012121 and 523157, NOXA1 and IDBG-93355 and ENSG00000188747 and 10811, NY-CO-31 and p51NOX and SDCCAG31, Noxa1 and IDBG-144012 and ENSMUSG00000036805 and 241275, Rac GTPase binding, this GO :0005515 and protein binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0006801 and superoxide metabolic process and biological process this GO :0010310 and regulation of hydrogen peroxide metabolic process and biological process this GO :0016176 and superoxide-generating NADPH oxidase activator activity and molecular function this GO :0017124 and SH3 domain binding and molecular function this GO :0019899 and enzyme binding and molecular function this GO :0043020 and NADPH oxidase complex and cellular component this GO :0043085 and positive regulation of catalytic activity and biological process this GO :0045087 and innate immune response and biological process this GO :0048365 and Rac GTPase binding and molecular function this GO :0060263 and regulation of respiratory burst and biological process, this GO :0005515 : protein binding, this GO :0005515 : protein binding and also this GO :0016176 : superoxide-generating NADPH oxidase activator activity and also this GO :0017124 : SH3 domain binding and also this GO :0019899 : enzyme binding and also this GO :0048365 : Rac GTPase binding, this GO :0016176 : superoxide-generating NADPH oxidase activator activity, this GO :0017124 : SH3 domain binding, this GO :0019899 : enzyme binding, this GO :0048365 : Rac GTPase binding, NADPH oxidase activator 1 |
| Identity: |
10668 |
| Gene: |
NOXA1 |
More about : NOXA1 |
| Long gene name: |
NADPH oxidase activator 1 |
| Synonyms gene: |
SDCCAG31 |
| Synonyms gene name: |
serologically defined colon cancer antigen 31 |
| Synonyms: |
NY-CO-31 FLJ25475 |
| Locus: |
9q34, 3 |
| Discovery year: |
1999-08-25 |
| GenBank acession: |
AF039697 |
| Entrez gene record: |
10811 |
| Pubmed identfication: |
9610721 |
| Havana BLAST/BLAT: |
OTTHUMG00000131781 |