| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
MEKK3 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Rat , we cannot guarantee that no other cross reactivity can occur, Mouse (Mus musculus) |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Titration of the MEKK3 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This MEKK3 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q61084 |
| Purity: |
Antigen affinity |
| Description: |
2007), By yeast 2-hybrid analysis of a mouse T-cell cDNA library, MAP3K3 is contiguously distal to LYK5 on chromosome 17(Puffenberger et al, Mekk3 and Osm colocalized in the cytoplasmic compartment of cotransfected cells, Protein interaction assays showed that Osm interacted directly with the Mekk3 substrate Mkk3(MAP2K3), Uhlik et al, Uhlik et al, also known as MAP/ERK kinase kinase 3 (Mekk3), and the Mekk3-Osm complex was recruited to Rac1 and cytoskeletal actin-containing membrane ruffles in response to sorbitol treatment, and with both GDP- and GTP-loaded Rac1, is an enzyme that in humans is encoded by the MAP3K3 gene, or MAPKKK3, which activates p38 in response to sorbitol-induced hyperosmotic conditions, with actin, (2003) concluded that the RAC1-OSM-MEKK3-MKK3 complex is required for regulation of p38 activity in response to osmotic shock, (2003) showed that a C-terminal fragment of mouse Osm(CCM2) interacted with MAP3K3/Mekk3, MAP3K3 (Mitogen-activated protein kinase kinase kinase 3) |
| Immunogen: |
An amino acid sequence from the N-terminus of mouse MAP3K3 (MKDLVALQMSRRTRL) was used as the immunogen for this MEKK3 antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the MEKK3 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
MEKK3 |
| Short name: |
MEKK3 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
MEKK3 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
6855 |
| Gene: |
MAP3K3 |
More about : MAP3K3 |
| Long gene name: |
mitogen-activated protein kinase kinase kinase 3 |
| Synonyms gene: |
MEKK3 |
| Synonyms: |
MAPKKK3 |
| Synonyms name: |
MAP/ERK kinase kinase 3 MAPK/ERK kinase kinase 3 |
| Locus: |
17q23, 3 |
| Discovery year: |
1997-11-14 |
| GenBank acession: |
U78876 |
| Entrez gene record: |
4215 |
| Pubmed identfication: |
9006902 |
| RefSeq identity: |
NM_002401 |
| Classification: |
Mitogen-activated protein kinase kinase kinases |
| Havana BLAST/BLAT: |
OTTHUMG00000178905 |