| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
FAP |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-F, IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, 5-1ug/ml, IHC (Frozen): 0, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Titration of the FAP antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This FAP antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q12884 |
| Purity: |
Antigen affinity |
| Description: |
Alpha, Depletion of FAP-expressing cells, FAP is most closely related to DPPIV and they share about 50% of their amino acids, FAPalpha shows 48% amino acid identity with dipeptidyl peptidase IV and 30% identity with DPP4-related protein, It is catalytically active as a 170kD dimer and has dipeptidase and gelatinase activity, Its gelatinase activity requires a glycine in P2 position, Northern blot analysis detected a 2, The FAP gene is mapped on 2q24, The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family, also known as FAPA or SEPRASE, caused rapid hypoxic necrosis of both cancer and stromal cells in immunogenic tumors by a process involving interferon-gamma and tumor necrosis factor-alpha, is an inducible cell surface glycoprotein that was originally identified in cultured fibroblasts using monoclonal antibody F19, which made up only 2% of all tumor cells in established Lewis lung carcinomas, 2, 8-kb FAPalpha mRNA in fibroblasts, Fibroblast Activation Protein |
| Immunogen: |
alpha (FFPNWISGQEYLHQSAD) was used as the immunogen for this FAP antibody, An amino acid sequence from the N-terminus of human Fibroblast activation protein |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the FAP antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
FAP |
| Short name: |
FAP Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
FAP (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
3590 |
| Gene: |
FAP |
More about : FAP |
| Long gene name: |
fibroblast activation protein alpha |
| Synonyms gene name: |
alpha , fibroblast activation protein |
| Synonyms: |
DPPIV |
| Synonyms name: |
seprase |
| Locus: |
2q24, 2 |
| Discovery year: |
1995-03-28 |
| GenBank acession: |
U09278 |
| Entrez gene record: |
2191 |
| Pubmed identfication: |
9247085 14707457 |
| Classification: |
DASH family |
| Havana BLAST/BLAT: |
OTTHUMG00000153890 |