| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
p130 RBL2 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the p130 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This p130 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q08999 |
| Purity: |
Antigen affinity |
| Description: |
Based on known syntenic relationships among human, Deletions of chromosome 16q have been found in several human neoplasms, In contrast, It is also known as Retionblastoma-related gene (RB2) and p130, Kong et al, Williams et al, Yeung et al, and prostate cancers, hepatic, including breast, ovarian, p107(RBL1) -/- and p130(RBL2) -/- fibroblasts were more susceptible to HRAS-mediated transformation than wildtype cells, rat and mouse, respectively, the data suggested that the mouse homolog resides on chromosome 8, using fluorescence in situ hybridization and somatic hybrid cell analysis, which supports the involvement of RBL2/p130 in human cancer as a tumor suppressor gene, with loss of either protein leading to longer telomeres, (1993) mapped the RBL2 gene to human chromosome 16q12, (2006) found that mouse fibroblasts lacking Rb were less susceptible to an oncogenic HRAS allele than wildtype cells, (2006)found that RBL2 and RINT1 were essential for telomere length control in human fibroblasts, 2 and rat chromosome 19, Retinoblastoma-like protein 2 is a protein that in humans is encoded by the RBL2 gene |
| Immunogen: |
An amino acid sequence from the C-terminus of human p130 (ENHSALLRRLQDVANDRGSH) was used as the immunogen for this p130 antibody (100% rat homology) |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the p130 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
p130 RBL2 |
| Short name: |
p130 Antibody RBL2 |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
p130 (Antibody to) RBL2 |
| Alternative technique: |
antibodies |
| Identity: |
9894 |
| Gene: |
RBL2 |
More about : RBL2 |
| Long gene name: |
RB transcriptional corepressor like 2 |
| Synonyms gene name: |
retinoblastoma-like 2 |
| Synonyms: |
Rb2 p130 |
| Locus: |
16q12, 2 |
| Discovery year: |
1994-01-17 |
| GenBank acession: |
X74594 |
| Entrez gene record: |
5934 |
| Pubmed identfication: |
8361765 8643454 |
| RefSeq identity: |
NM_005611 |
| Havana BLAST/BLAT: |
OTTHUMG00000133198 |