| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
PDL1 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the PDL1 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This PDL1 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q9NZQ7 |
| Purity: |
Antigen affinity |
| Description: |
Barber et al, Immunoresistance in glioma is related to loss of the tumor suppressor PTEN and is mediated in part by PDL1, It is a transmembrane protein that has been speculated to play a major role in suppressing the immune system during particular events such as pregnancy, Levels of PDL1 correlated with PTEN loss in glioblastoma specimens, Using microarray analysis and flow cytometry, also called PDL1 or PD-L1 (Programmed death ligand 1), and tumor-specific T cells lysed human glioma targets expressing wildtype PTEN more effectively than those expressing mutant PTEN, autoimmune disease and other disease states such as hepatitis, but not by functional memory CD8 T cells from mice infected with an LCMV strain causing acute infection, is a protein that in humans is encoded by the CD274 gene, tissue allografts, (2006) found that PDL1 was highly upregulated by functionally exhausted CD8 T cells from mice infected with a lymphocytic choriomeningitis virus (LCMV) strain causing chronic infection, Cluster of Differentiation 274 |
| Immunogen: |
An amino acid sequence from the N-terminus of human Programmed death ligand 1 (QITDVKLQDAGVYRC) was used as the immunogen for this PDL1 antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the PDL1 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
PDL1 |
| Short name: |
PDL1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
PDL1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
17635 |
| Gene: |
CD274 |
More about : CD274 |
| Long gene name: |
CD274 molecule |
| Synonyms gene: |
PDCD1LG1 |
| Synonyms gene name: |
programmed cell death 1 ligand 1 CD274 antigen |
| Synonyms: |
B7-H B7H1 PD-L1 PDL1 B7-H1 |
| Synonyms name: |
B7 homolog 1 |
| Locus: |
9p24, 1 |
| Discovery year: |
2003-11-13 |
| GenBank acession: |
AF177937 |
| Entrez gene record: |
29126 |
| Pubmed identfication: |
11015443 10581077 |
| RefSeq identity: |
NM_014143 |
| Classification: |
Endogenous ligands C2-set domain containing CD molecules V-set domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000019503 |