| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
BiP GRP78 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ICC, IHC-F, IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, 5-1ug/ml, 5-1ug/ml, IHC (Frozen): 0, IHC (Paraffin): 0, Immunocytochemistry: 0, Western blot: 0 |
| Notes: |
Titration of the BiP antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting points |
| Intented use: |
This BiP antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P11021 |
| Purity: |
Antigen affinity |
| Description: |
78kD(GRP78) or BiP, Shen et al, The findings ofShen et al, as well as playing a role in retrograde transport across the ER membrane of aberrant proteins destined for degradation by theproteasome, is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum, (2002)concluded thatBiPretains ATF6 in the ER by inhibiting its Golgi localization signals and that dissociation ofBiPduring ER stress allows ATF6 to be transported to the Golgi, (2002)demonstrated thatthe proteinis a key element in sensing the folding capacity within the ER, BiP is an essential component of the translocation machinery, HSPA5 (heat shock 70kDa protein 5) also known as glucose-regulated protein |
| Immunogen: |
mouse and rat), An amino acid sequence from the C-terminus of human GRP78/BiP (EWLESHQDADIEDFK) was used as the immunogen for this BiP antibody (100% homologous in human |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the BiP antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
BiP GRP78 |
| Short name: |
BiP Antibody GRP78 |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
BiP (Antibody to) GRP78 |
| Alternative technique: |
antibodies |
| Identity: |
5238 |
| Gene: |
HSPA5 |
More about : HSPA5 |
| Long gene name: |
heat shock protein family A (Hsp70) member 5 |
| Synonyms gene: |
GRP78 |
| Synonyms gene name: |
78kD) heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) , heat shock 70kD protein 5 (glucose-regulated protein |
| Synonyms: |
BiP |
| Synonyms name: |
78kDa , glucose-regulated protein |
| Locus: |
9q33, 3 |
| Discovery year: |
1991-07-26 |
| Entrez gene record: |
3309 |
| Classification: |
Heat shock 70kDa proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000020672 |