| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
E2F2 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the E2F2 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This E2F2 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q14209 |
| Purity: |
Antigen affinity |
| Description: |
Electrophoretic mobility shift assays revealed the specific binding of E2F2 to ECE1b promoter sequences containing either allele of the C-338A polymorphism, In contrast, The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses, The ability of Myc to induce S phase was impaired in the absence of either E2f2 or E2f3 but not E2f1 or E2f4, The gene is mapped to 1p36 by fluorescence in situ hybridization, also called E2F-2, is a member of the E2F family of transcription factors, the ability of Myc to induce apoptosis was markedly reduced in cells deleted for E2f1 but not E2f2 or E2f3, with the -338A allele being associated with an increased affinity to E2F2 compared with -338C, E2F transcription factor 2 |
| Immunogen: |
An amino acid sequence from the C-terminus of human E2F transcription factor 2 (ISDLFDSYDLGDLLIN) was used as the immunogen for this E2F2 antibody (100% mouse homology) |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the E2F2 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
E2F2 |
| Short name: |
E2F2 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
E2F transcription factor 2 (Antibody to) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
DNA-templated and biological process this GO :0006367 and transcription initiation from RNA polymerase II promoter and biological process this GO :0008134 and transcription factor binding and molecular function this GO :0051726 and regulation of cell cycle and biological process this GO :0072332 and intrinsic apoptotic signaling pathway by p53 class mediator and biological process this GO :1990086 and lens fiber cell apoptotic process and biological process, E2F-2, E2F2 and IDBG-645457 and ENSBTAG00000014400 and 617024, E2F2 and IDBG-93950 and ENSG00000007968 and 1870, E2f2 and IDBG-197705 and ENSMUSG00000018983 and 242705, nuclei, this GO :0000278 and mitotic cell cycle and biological process this GO :0001047 and core promoter binding and molecular function this GO :0003677 and DNA binding and molecular function this GO :0003700 and sequence-specific DNA binding transcription factor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005667 and transcription factor complex and cellular component this GO :0006355 and regulation of transcription, this GO :0001047 : core promoter binding, this GO :0001047 : core promoter binding and also this GO :0003677 : DNA binding and also this GO :0003700 : sequence-specific DNA binding transcription factor activity and also this GO :0005515 : protein binding and also this GO :0008134 : transcription factor binding, this GO :0003677 : DNA binding, this GO :0003700 : sequence-specific DNA binding transcription factor activity, this GO :0005515 : protein binding, this GO :0008134 : transcription factor binding, transcription factor binding, E2F transcription factor 2 |
| Identity: |
3114 |
| Gene: |
E2F2 |
More about : E2F2 |
| Long gene name: |
E2F transcription factor 2 |
| Synonyms: |
E2F-2 |
| Locus: |
1p36, 12 |
| Discovery year: |
1994-08-31 |
| GenBank acession: |
L22846 |
| Entrez gene record: |
1870 |
| Pubmed identfication: |
8246995 8246996 |
| RefSeq identity: |
NM_004091 |
| Classification: |
E2F transcription factors |
| Havana BLAST/BLAT: |
OTTHUMG00000003223 |