| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
MAG |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml (1), Western blot: 0 |
| Notes: |
1, MAG is seen in western blotting from 60~100KD depending on the level of glycosylation, Titration of the MAG antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting points |
| Intented use: |
This MAG antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P20916 |
| Purity: |
Antigen affinity |
| Description: |
Cleavage of GPI-linked proteins from axons protects growth cones from MAG-induced collapse, Experiments blocking NgR from interacting with the glycoprotein prevented inhibition of neurite outgrowth by MAG, In cultured human embryonic kidney (HEK) cells expressing the NOGO receptor, It is though to be involved in the process of myelination, NgR, Resistant embryonic neurons were rendered sensitive by expression of NgR, The glycoprotein binds specifically to an NgR-expressing cell line in a GPI-dependent and sialic acid-independent manner, also known as SIGLEC4A, and dominant-negative NgR eliminates MAG inhibition of neurite outgrowth, is a cell membrane glycoprotein that is a member of the SIGLEC family of proteins and is a functional ligand of the NOGO-66 receptor, p75(NTR) was required for MAG-induced intracellular calcium elevation, Myelin-associated glycoprotein |
| Immunogen: |
mouse and rat), Amino acids 611-626 (YTLTEELAEYAEIRVK) were used as the immunogen for this MAG antibody (100% homologous in human |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the MAG antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
MAG |
| Short name: |
MAG Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
myelin associated glycoprotein (Antibody to) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
BT, GMA and S-MAG and SIGLEC-4A and SIGLEC4A, MAG and IDBG-43939 and ENSG00000105695 and 4099, Mag and IDBG-171670 and ENSMUSG00000036634 and 17136, Plasma membranes, protein binding, this GO :0005515 and protein binding and molecular function this GO :0005886 and plasma membrane and cellular component this GO :0007155 and cell adhesion and biological process this GO :0007596 and blood coagulation and biological process this GO :0016021 and integral component of membrane and cellular component this GO :0021762 and substantia nigra development and biological process this GO :0033270 and paranode region of axon and cellular component this GO :0043209 and myelin sheath and cellular component this GO :0043220 and Schmidt-Lanterman incisure and cellular component this GO :0048011 and neurotrophin TRK receptor signaling pathway and biological process this GO :0050770 and regulation of axonogenesis and biological process this GO :0050771 and negative regulation of axonogenesis and biological process this GO :0050900 and leukocyte migration and biological process, this GO :0005515 : protein binding, this GO :0005515 : protein binding, 96871 and IDBG-635591 and ENSBTAG00000017044 and 540576, myelin associated glycoprotein |
| Identity: |
6783 |
| Gene: |
MAG |
More about : MAG |
| Long gene name: |
myelin associated glycoprotein |
| Synonyms gene: |
GMA |
| Synonyms: |
SIGLEC4A SIGLEC-4A S-MAG |
| Synonyms name: |
sialic acid binding Ig-like lectin 4A |
| Locus: |
19q13, 1 |
| Discovery year: |
2001-06-22 |
| GenBank acession: |
M29273 |
| Entrez gene record: |
4099 |
| Pubmed identfication: |
2476987 8432525 |
| RefSeq identity: |
NM_080600 |
| Classification: |
C2-set domain containing I-set domain containing Sialic acid binding Ig like lectins |
| Havana BLAST/BLAT: |
OTTHUMG00000183296 |