| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
CD146 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Titration of the CD146 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This CD146 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P43121 |
| Purity: |
Antigen affinity |
| Description: |
By radiation hybrid analysis, CD146 has been demonstrated to appear on a small subset of T and B lymphocytes in the peripheral blood of healthy individuals, CD146 has been seen as a marker for mesenchymal stem cells isolated from multiple adult and fetal organs, In humans, It is a member of the immunoglobulin superfamily, Kuske and Johnson(1999) mapped the gene to chromosome 11q23, The CD146+ T cells display an immunophenotype consistent with effector memory cells and have a distinct gene profile from the CD146- T cells, also known as the melanoma cell adhesion molecule (MCAM) or cell surface glycoprotein MUC18, and its expression may be linked to multipotency, is a glycosylated 113kDa cell adhesion molecule used as a marker for endothelial cell lineage, is homologous to several cell adhesion molecules and is associated with tumor progression and the development of metastasis in human malignant melanoma, mesenchymal stem cells with greater differentiation potential express higher levels of the protein on the cell surface, the CD146 protein is encoded by the MCAM gene, 3, Cluster of differentiation 146 |
| Immunogen: |
An amino acid sequence from the C-terminus of human CD146 (KKGKLPCRRSGKQEITL) was used as the immunogen for this CD146 antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the CD146 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
CD146 |
| Short name: |
CD146 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
CD146 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
6934 |
| Gene: |
MCAM |
More about : MCAM |
| Long gene name: |
melanoma cell adhesion molecule |
| Synonyms: |
MUC18 CD146 |
| Synonyms name: |
Gicerin |
| Locus: |
11q23, 3 |
| Discovery year: |
1995-12-18 |
| GenBank acession: |
X68264 |
| Entrez gene record: |
4162 |
| Pubmed identfication: |
2602381 10702685 |
| Classification: |
C2-set domain containing CD molecules V-set domain containing Immunoglobulin like domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000166197 |