| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
GAT-1 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Titration of the GAT-1 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This GAT-1 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P30531 |
| Purity: |
Antigen affinity |
| Description: |
By in vitro functional expression studies, GABATHG and GAT-1, Gaba), Member 1), Studies of postmortem hippocampus from 69 African American individuals showed that overall expression decreased with age, Zomot et al, also called GABATR, encodes a gamma-aminobutyric acid (GABA) transporter, the GAT-1 insertion promoter (two copies of the sequence) had significantly greater promoter activity than the noninsertion (one copy of the sequence) promoter, which in turn had greater activity than a promoterless control, which removes GABA from the synaptic cleft, (2007) showed that introduction of a negatively charged amino acid at or near one of the two putative sodium binding sites of GAT-1 from rat brain renders both net flux and exchange of GABA largely chloride independent, SLC6A1 (Solute Carrier family 6(Neurotransmitter transporter |
| Immunogen: |
mouse and rat), An amino acid sequence from the C-terminus of human GABA Transporter 1 (WFYGVNRFYDNIQEM) was used as the immunogen for this GAT-1 antibody (100% homologous in human |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the GAT-1 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
GAT-1 |
| Short name: |
GAT-1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
GAT-1 (Antibody to) |
| Alternative technique: |
antibodies |