| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
PBP |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Rat , we cannot guarantee that no other cross reactivity can occur, Mouse (Mus musculus) |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Titration of the PBP antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This PBP antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P70296 |
| Purity: |
Antigen affinity |
| Description: |
Depletion of the protein decreased the mitotic index, Overexpression interferes with the activation of MEK and ERK, PBP coimmunoprecipitates with RAF1 and MEK from cell lysates and colocalizes with RAF1 when examined by confocal microscopy, PBP expression was rapidly upregulated during induction of chemotherapy-triggered apoptosis in human prostate and breast cancer cell lines, and an override of mitotic checkpoints induced by spindle poisons, and maximal expression correlated perfectly with the onset of apoptosis by Chatterjee et al(2004), and transformation elicited by an oncogenically activated RAF1 kinase, induction of AP1-dependent reporter genes, the number of metaphase cells, traversal times from nuclear envelope breakdown to anaphase, Phosphatidylethanolamine-binding protein inhibits the phosphorylation and activation of MEK by RAF1 |
| Immunogen: |
An amino acid sequence from the N-terminus of mouse PBP (QEVDEPPQHALRVDY) was used as the immunogen for this PBP antibody (100% rat homology) |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the PBP antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
PBP |
| Short name: |
PBP Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
PBP (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
2989 |
| Gene: |
DOCK3 |
More about : DOCK3 |
| Long gene name: |
dedicator of cytokinesis 3 |
| Synonyms gene name: |
dedicator of cyto-kinesis 3 |
| Synonyms: |
KIAA0299 MOCA PBP |
| Locus: |
3p21, 2 |
| Discovery year: |
1998-05-13 |
| GenBank acession: |
AB002297 |
| Entrez gene record: |
1795 |
| Pubmed identfication: |
9205841 |
| RefSeq identity: |
NM_004947 |
| Havana BLAST/BLAT: |
OTTHUMG00000156892 |