| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
FGF21 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the FGF21 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This FGF21 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q9NSA1 |
| Purity: |
Antigen affinity |
| Description: |
Changes in expression due to suckling or nutritional manipulations were associated with changes in circulating free fatty acid and ketone body levels, FGF21 also protects animals from diet-induced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to diabetic rodents, FGF21 stimulates glucose uptake in adipocytes but not in other cell types, FGF21 treatment of adipocytes is associated with phosphorylation of FRS2, Fgf21 treatment increased the expression of thermogenic genes, In differentiated mouse brown adipocytes in culture, The level of expression in adipose tissue was comparable to that in liver, This effect is additive to the activity of insulin, Using RT-PCR, a member of the fibroblast growth factor(FGF) family, a protein linking FGF receptors to the Ras/MAP kinase pathway, and enhanced glucose oxidation, caused higher total and uncoupled respiration, including subcutaneous and epididymal fat pads and brown adipose tissue, the protein was found to express in several types of adipose tissue in mice, Fibroblast growth factor 21 is a protein that in humans is encoded by the FGF21 gene |
| Immunogen: |
Amino acids 43-58 (QVRQRYLYTDDAQQTE-human) were used as the immunogen for this FGF21 antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the FGF21 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
FGF21 |
| Short name: |
FGF21 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
FGF21 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
3678 |
| Gene: |
FGF21 |
More about : FGF21 |
| Long gene name: |
fibroblast growth factor 21 |
| Locus: |
19q13, 33 |
| Discovery year: |
1999-11-26 |
| GenBank acession: |
AB021975 |
| Entrez gene record: |
26291 |
| Pubmed identfication: |
10858549 |
| Havana BLAST/BLAT: |
OTTHUMG00000183326 |