| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
IKKa |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the IKKa antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This IKKa antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
O15111 |
| Purity: |
Antigen affinity |
| Description: |
By FISH, I-KAPPA-B, IKK1, IKKA, IKKa is expressed in a broad array of tissues and high degree of conservation across species, IKKa is part of the I kappa B kinase complex that plays an important role in regulating the NF-kappa B transcription factor, It is a member of the protein kinase superfamily, NFKBIKA, Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA or IKBKB) marks them for destruction via the ubiquitination pathway, The protein contains a serine/threonine kinase domain, also called CHUK, is a protein kinase that in humans is encoded by the CHUK gene, the IKBKA gene is mapped to chromosome 10q24, thereby allowing activation of the NF-kappa-B complex, IKBKA |
| Immunogen: |
An amino acid sequence from the C-terminus of human IKK alpha (NEEQGNSMMNLDWSWLTE) was used as the immunogen for this IKKa antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the IKKa antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
IKKa |
| Short name: |
IKKa Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
IKKa (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
1974 |
| Gene: |
CHUK |
More about : CHUK |
| Long gene name: |
conserved helix-loop-helix ubiquitous kinase |
| Synonyms gene: |
TCF16 |
| Synonyms: |
IKK1 IKK-alpha IkBKA NFKBIKA IKKA |
| Locus: |
10q24, 31 |
| Discovery year: |
1995-03-15 |
| GenBank acession: |
AF009225 |
| Entrez gene record: |
1147 |
| Pubmed identfication: |
7558004 16902410 |
| RefSeq identity: |
NM_001278 |
| Havana BLAST/BLAT: |
OTTHUMG00000018899 |