| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
FAK |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml (1), Western blot: 0 |
| Notes: |
1, FAK/PTK2 has numerous isoforms and is seen in western blot between 80 ~ 110KD, Titration of the FAK antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting points |
| Intented use: |
This FAK antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q05397 |
| Purity: |
Antigen affinity |
| Description: |
FAK activity elicits intracellular signal transduction pathways that promote the turn-over of cell contacts with the extracellular matrix, FAK is not absolutely required for cell migration, It has been shown that when the protein was blocked, It is a member of the FAK subfamily of tyrosine kinases that included PYK2 but lacks significant sequence similarity to kinases from other subfamilies, It is required during development, Its tyrosine kinase activity can be activated, PTK2 is a focal adhesion-associated protein kinase involved in cellular adhesion (how cells stick to each other and their surroundings) and movement, This gene encodes a cytosolic tyrosine kinase that is found concentrated in the focal adhesions that form among cells attaching to extracellular matrix constituents, With the exception of certain types of blood cells, also known as PTK2 (protein tyrosine kinase 2) is encoded by the PTK2 gene, and loss of the protein results in lethality, and may play other roles in the cell, breast cancer cells became less metastastic due to decreased mobility, including the regulation of the tumor suppressor p53, most cells express the protein, promoting cell migration, which plays a key important early step in cell migration, Focal Adhesion Kinase |
| Immunogen: |
mouse and rat), Amino acids 1036-1052 (DVIDQARLKMLGQTRPH) were used as the immunogen for this FAK antibody (100% homologous in human |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the FAK antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
FAK |
| Short name: |
FAK Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
FAK (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
9611 |
| Gene: |
PTK2 |
More about : PTK2 |
| Long gene name: |
protein tyrosine kinase 2 |
| Synonyms gene name: |
PTK2 protein tyrosine kinase 2 |
| Synonyms: |
FAK FADK FAK1 PPP1R71 |
| Synonyms name: |
protein phosphatase 1, regulatory subunit 71 |
| Locus: |
8q24, 3 |
| Discovery year: |
1992-10-02 |
| GenBank acession: |
L13616 |
| Entrez gene record: |
5747 |
| Pubmed identfication: |
8422239 |
| RefSeq identity: |
NM_005607 |
| Classification: |
Protein phosphatase 1 regulatory subunits FERM domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000067438 |