| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
APEX1 (APE1) |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Titration of the APEX1 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This APE1 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P27695 |
| Purity: |
Antigen affinity |
| Description: |
The abundance of the large form of the protein was increased in leiomyoma extracts relative to myometrial tissue extracts, The exonuclease activity of nuclear APEX1 can remove the anti-HIV nucleoside analogs AZT and D4T from the 3-prime terminus of a nick more efficiently than can cytosolic exonucleases, and the large form was dominant in cell lines derived from leiomyosarcomas, or Apurinic endonuclease 1 (APE1), was identified as a member of a family of DNA repair enzymes found in lower organisms, which contains probable nuclear transport signals, APEX1 |
| Immunogen: |
mouse and rat), An amino acid sequence from the middle region of human APEX1 (RGLVRLEYRQRWDEA) was used as the immunogen for this APEX1 antibody (100% homologous in human |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the APEX1 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
APEX1 (APE1) |
| Short name: |
APEX1 Antibody (APE1) |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
APEX1 (Antibody to) (APE1) |
| Alternative technique: |
antibodies |
| Identity: |
587 |
| Gene: |
APEX1 |
More about : APEX1 |
| Long gene name: |
apurinic/apyrimidinic endodeoxyribonuclease 1 |
| Synonyms gene: |
APEX |
| Synonyms gene name: |
APEX nuclease (multifunctional DNA repair enzyme) APEX nuclease (multifunctional DNA repair enzyme) 1 |
| Synonyms: |
APE REF1 HAP1 APX APEN REF-1 APE-1 |
| Locus: |
14q11, 2 |
| Discovery year: |
1997-05-22 |
| GenBank acession: |
X59764 |
| Entrez gene record: |
328 |
| RefSeq identity: |
NM_001641 |
| Havana BLAST/BLAT: |
OTTHUMG00000029544 |
| Locus Specific Databases: |
ALSOD, the Amyotrophic Lateral Sclerosis Online Genetic Database |