| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
BAK |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ICC, IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Immunocytochemistry: 0, Western blot: 0 |
| Notes: |
Titration of the BAK antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This BAK antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q16611 |
| Purity: |
Antigen affinity |
| Description: |
BAK also interacts with the tumor suppressor P53 after exposure to cell stress, By Southern blot analysis of genomic DNA from human/rodent somatic cell hybrids, It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, The gene spans 7, The protein is a pro-apoptotic member of the Bcl-2 gene family which is involved in initiating apoptosis, This protein localizes to mitochondria, and functions to induce apoptosis, encoded by the BAK gene, the gene is localized to chromosome 6, which leads to a loss in membrane potential and the release of cytochrome, 6 kb and contains 6 exons, Bcl2 antagonist killer is a protein that in humans |
| Immunogen: |
An amino acid sequence from the middle region of human BAK (QVGRQLALIGDDINRRYDSEFQ) was used as the immunogen for this BAK antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the BAK antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
BAK |
| Short name: |
BAK Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
BAK (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
949 |
| Gene: |
BAK1 |
More about : BAK1 |
| Long gene name: |
BCL2 antagonist/killer 1 |
| Synonyms gene: |
CDN1 |
| Synonyms gene name: |
BCL2-antagonist/killer 1 |
| Synonyms: |
BCL2L7 BAK |
| Locus: |
6p21, 31 |
| Discovery year: |
1997-10-09 |
| GenBank acession: |
U23765 |
| Entrez gene record: |
578 |
| Pubmed identfication: |
7715730 7715731 |
| RefSeq identity: |
NM_001188 |
| Classification: |
BCL2 family |
| Havana BLAST/BLAT: |
OTTHUMG00000014530 |