| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Osteopontin |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Rat , we cannot guarantee that no other cross reactivity can occur, Mouse (Mus musculus) |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Titration of the Osteopontin antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting points |
| Intented use: |
This Osteopontin antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P10923 |
| Purity: |
Antigen affinity |
| Description: |
It is a phosphorylated glycoprotein secreted to the mineralizing extracellular matrix by osteoblasts during bone development, Osteopontin is presumably involved in stone formation as a stone matrix protein, and early T lymphocyte activation 1(ETA1), bone sialoprotein, secreted phosphoprotein 1(SPP1), Osteopontin (OPN) is also called urinary stone protein |
| Immunogen: |
Amino acids 268-285 (DPKSKEDDRYLKFRISHE-mouse) were used as the immunogen for this Osteopontin antibody (100% rat homology) |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the Osteopontin antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Osteopontin |
| Short name: |
Osteopontin Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
Osteopontin (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
8151 |
| Gene: |
OPN |
More about : OPN |
| Long gene name: |
secreted phosphoprotein 1 |
| Synonyms gene: |
BNSP |
| Synonyms gene name: |
osteopontin bone sialoprotein I |
| Synonyms: |
BSPI ETA-1 SPP1 |
| Synonyms name: |
early T-lymphocyte activation 1 |
| Locus: |
4q22, 1 |
| Discovery year: |
1989-03-06 |
| Entrez gene record: |
6696 |
| Pubmed identfication: |
1575754 |
| Classification: |
Endogenous ligands SIBLING family |
| Havana BLAST/BLAT: |
OTTHUMG00000130599 |