| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
IL-6 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Rat , we cannot guarantee that no other cross reactivity can occur, Mouse (Mus musculus) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the IL-6 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting points |
| Intented use: |
This IL-6 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P20607 |
| Purity: |
Antigen affinity |
| Description: |
Bowcock et al, By in situ hybridization and Southern blot analysis of mouse-human hybrid cell lines, IL-6 is also essential for hybridoma growth and is found in many supplemental cloning media such as briclone, IL-6 is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine, IL-6 is one of the most important mediators of fever and of the acute phase response, It is secreted by T cells and macrophages to stimulate immune response to trauma, Sutherland et al, especially burns or other tissue damage leading to inflammation, (1988) assigned the IL6 gene to chromosome 7p21, (1988) mapped the IL6 gene to chromosome 7p15, Interleukin-6(IL-6) is a protein that in humans is encoded by the IL6 gene |
| Immunogen: |
Amino acids 195-211 (KALEEFLKVTMRSTRQT) were used as the immunogen for this IL-6 antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the IL-6 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
IL-6 |
| Short name: |
IL-6 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
Interleukin-6 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
6018 |
| Gene: |
IL6 |
More about : IL6 |
| Long gene name: |
interleukin 6 |
| Synonyms gene: |
IFNB2 |
| Synonyms gene name: |
beta 2) , interleukin 6 (interferon |
| Synonyms: |
IL-6 BSF2 HGF HSF |
| Synonyms name: |
beta 2 , interferon |
| Locus: |
7p15, 3 |
| Discovery year: |
1986-01-01 |
| GenBank acession: |
M18403 |
| Entrez gene record: |
3569 |
| Pubmed identfication: |
3294161 |
| RefSeq identity: |
NM_000600 |
| Classification: |
Interleukins Interferons Interleukin 6 cytokine family |
| Havana BLAST/BLAT: |
OTTHUMG00000023178 |