| Category: |
Antibody |
| Concentration: |
025% sodium azide, 2mg/ml in 1X PBS with 50% glycerol and 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Actin |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Titration of the Actin antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting points |
| Intented use: |
This Actin antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P63261 |
| Purity: |
Antigen affinity |
| Description: |
Because the amino acid sequence of the C-terminal is the same for almost all actins, It varies in amount, The actins exhibit over 90% sequence homology, The isoforms are comprised of three alpha-actin, a highly conserved protein, being related to the type of differentiation and to the functional state of cells and tissues, but each isoform has a unique NH2-terminal sequence, is a major component of both the cytoskeletal and contractile structures in the cell types, one beta-actin, this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues, two gamma-actin, Actin |
| Immunogen: |
mouse and rat), An amino acid sequence from the C-terminus of the human gamma isoform (SGPSIVHRKCF) was used as the immunogen for this Actin antibody (100% homologous human |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Store the Actin antibody at -20 deg |
| Localization: |
Cytoplasmic |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Actin |
| Short name: |
Actin Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
Actin (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
14082 |
| Gene: |
ANLN |
More about : ANLN |
| Long gene name: |
anillin actin binding protein |
| Synonyms gene name: |
Drosophila) , actin binding protein (scraps homolog, actin binding protein anillin, anillin (Drosophila Scraps homolog) |
| Synonyms: |
ANILLIN Scraps scra |
| Locus: |
7p14, 2 |
| Discovery year: |
2001-01-03 |
| GenBank acession: |
AF273437 |
| Entrez gene record: |
54443 |
| Pubmed identfication: |
10931866 |
| RefSeq identity: |
NM_018685 |
| Classification: |
Pleckstrin homology domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000023143 |