| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
HRH3 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the HRH3 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This HRH3 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q9Y5N1 |
| Purity: |
Antigen affinity |
| Description: |
Additionally, And it shares 22% and 21, HRH3 was most notably observed throughout the thalamus, Strong expression was also seen in layers II, The deduced 445-amino acid HRH3 protein contains 7 predicted transmembrane domains, The expression of recombinant HRH3 in a variety of cell lines conferred an ability to inhibit adenylate cyclase in response to histamine, V, and VIb of the cerebral cortex, and in olfactory tubercle, and the caudate nucleus, but not to acetylcholine or any other biogenic amine, in the pyramidal layers of the hippocampus, respectively, the ventromedial hypothalamus, 4% amino acid sequence homology with the H1(HRH1) and H2(HRH2) receptors, The histamine receptor H3(HRH3) is a presynaptic autoreceptor on histamine neurons in the brain and a presynaptic heteroreceptor in nonhistamine-containing neurons in both the central and peripheral nervous systems |
| Immunogen: |
An amino acid sequence from the C-terminus of human HRH3 (CPQKLKIQPHSSLEHCWK) was used as the immunogen for this HRH3 antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the HRH3 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
HRH3 |
| Short name: |
HRH3 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
histamine receptor H3 (Antibody to) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
GPCR97 and HH3R, HRH3 and IDBG-641005 and ENSBTAG00000031625 and 789851, HRH3 and IDBG-84247 and ENSG00000101180 and 11255, Hrh3 and IDBG-213755 and ENSMUSG00000039059 and 99296, Plasma membranes, coupled to cyclic nucleotide second messenger and biological process this GO :0007194 and negative regulation of adenylate cyclase activity and biological process this GO :0007204 and positive regulation of cytosolic calcium ion concentration and biological process this GO :0007269 and neurotransmitter secretion and biological process this GO :0007420 and brain development and biological process this GO :0007612 and learning and biological process this GO :0007613 and memory and biological process this GO :0008144 and drug binding and molecular function this GO :0014050 and negative regulation of glutamate secretion and biological process this GO :0014053 and negative regulation of gamma-aminobutyric acid secretion and biological process this GO :0014061 and regulation of norepinephrine secretion and biological process this GO :0014063 and negative regulation of serotonin secretion and biological process this GO :0014070 and response to organic cyclic compound and biological process this GO :0016021 and integral component of membrane and cellular component this GO :0042755 and eating behavior and biological process this GO :0042756 and drinking behavior and biological process this GO :0043005 and neuron projection and cellular component this GO :0043209 and myelin sheath and cellular component this GO :0045776 and negative regulation of blood pressure and biological process this GO :0050679 and positive regulation of epithelial cell proliferation and biological process this GO :0050890 and cognition and biological process, drug binding, this GO :0004930 and G-protein coupled receptor activity and molecular function this GO :0004969 and histamine receptor activity and molecular function this GO :0005886 and plasma membrane and cellular component this GO :0005887 and integral component of plasma membrane and cellular component this GO :0007186 and G-protein coupled receptor signaling pathway and biological process this GO :0007187 and G-protein coupled receptor signaling pathway, this GO :0004930 : G-protein coupled receptor activity, this GO :0004930 : G-protein coupled receptor activity and also this GO :0004969 : histamine receptor activity and also this GO :0008144 : drug binding, this GO :0004969 : histamine receptor activity, this GO :0008144 : drug binding, histamine receptor H3 |
| Identity: |
5184 |
| Gene: |
HRH3 |
More about : HRH3 |
| Long gene name: |
histamine receptor H3 |
| Synonyms: |
GPCR97 |
| Locus: |
20q13, 33 |
| Discovery year: |
2000-01-07 |
| GenBank acession: |
AF140538 |
| Entrez gene record: |
11255 |
| Pubmed identfication: |
10347254 |
| RefSeq identity: |
NM_007232 |
| Classification: |
Histamine receptors |
| Havana BLAST/BLAT: |
OTTHUMG00000032899 |