| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Connexin 43 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Titration of the Connexin 43 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This Connexin 43 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P17302 |
| Purity: |
Antigen affinity |
| Description: |
Connexin 43, Cx43 also plays a critical role in the physiology of hearing, Mutations in the gene, alpha-1(GJA1), also called GAP Junction Protein, are associated with visceroatrial heterotaxia, is a member of the connexin gene family which abundantly expressed in the heart and liver and was mapped to 6q21-q23, is targeted by several protein kinases that regulate myocardial cell-cell coupling, presumably by participating in the recycling of potassium to the cochlear endolymph, the major protein of gap junctions in the heart, which lead to abnormally regulated cell-cell communication, 2, Connexin 43 (Cx43) |
| Immunogen: |
An amino acid sequence from the C-terminus of human Connexin 43 (RASSRASSRPRPDDLEI) was used as the immunogen for this Connexin 43 antibody (100% conserved in mouse and rat) |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the Connexin 43 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Connexin 43 |
| Short name: |
Connexin 43 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
Connexin 43 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
4274 |
| Gene: |
GJA1 |
More about : GJA1 |
| Long gene name: |
gap junction protein alpha 1 |
| Synonyms gene: |
ODDD GJAL |
| Synonyms gene name: |
43kDa , 43kDa (connexin 43) gap junction protein, alpha 1, alpha 1, alpha-like gap junction protein, gap junction protein |
| Synonyms: |
CX43 ODD ODOD SDTY3 |
| Synonyms name: |
oculodentodigital dysplasia (syndactyly type III) connexin 43 |
| Locus: |
6q22, 31 |
| Discovery year: |
1990-08-03 |
| GenBank acession: |
BC026329 |
| Entrez gene record: |
2697 |
| Pubmed identfication: |
10331943 1646158 |
| RefSeq identity: |
NM_000165 |
| Classification: |
Gap junction proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000015479 |
| Locus Specific Databases: |
Hereditary Hearing Loss Homepage |