| Antigen detectable with this ELISA kit: |
Tartrate Resistant (ACP5), Acid Phosphatase 5 |
| Species reactivity: |
Rattus norvegicus (Rat) |
| Range of detection: |
0, 1, 25ng/mL, 3, 6, 12, 125ng/mL, 25ng/mL, 563ng/mL, 5ng/mL, 781-50ng/mL The standard curve concentrations used for the ELISA&rsquo, 781ng/mL, s were 50ng/mL |
| Sensitivity: |
34ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
plasma, tissue homogenates and other biological fluids, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Acid Phosphatase 5, Tartrate Resistant (ACP5), Tartrate Resistant (ACP5), Tartrate Resistant (ACP5), Tartrate Resistant (ACP5) in the samples is then determined by comparing the O, The concentration of Acid Phosphatase 5, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Acid Phosphatase 5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Acid Phosphatase 5, D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Tartrate Resistant (ACP5), Acid Phosphatase 5 |
| Short name: |
Tartrate Resistant (ACP5) ELISA kit, Acid Phosphatase 5 |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Tartrate Resistant (acid phosphatase 5, tartrate resistant) Enzyme-linked immunosorbent assay reagent, Acid Phosphatase 5 |
| Alternative technique: |
elisas |
| Alternative to gene target: |
ACP5 and IDBG-29654 and ENSG00000102575 and 54, ACP5 and IDBG-643580 and ENSBTAG00000004826 and 517002, Acp5 and IDBG-143414 and ENSMUSG00000001348 and 11433, Plasma membranes, SPENCDI and TRAP, hydrolase activity, tartrate resistant, this GO :0003993 and acid phosphatase activity and molecular function this GO :0005764 and lysosome and cellular component this GO :0005829 and cytosol and cellular component this GO :0006766 and vitamin metabolic process and biological process this GO :0006767 and water-soluble vitamin metabolic process and biological process this GO :0006771 and riboflavin metabolic process and biological process this GO :0008198 and ferrous iron binding and molecular function this GO :0008199 and ferric iron binding and molecular function this GO :0016021 and integral component of membrane and cellular component this GO :0016311 and dephosphorylation and biological process this GO :0016787 and hydrolase activity and molecular function this GO :0032496 and response to lipopolysaccharide and biological process this GO :0032691 and negative regulation of interleukin-1 beta production and biological process this GO :0032695 and negative regulation of interleukin-12 production and biological process this GO :0032720 and negative regulation of tumor necrosis factor production and biological process this GO :0032929 and negative regulation of superoxide anion generation and biological process this GO :0034097 and response to cytokine and biological process this GO :0044281 and small molecule metabolic process and biological process this GO :0045019 and negative regulation of nitric oxide biosynthetic process and biological process this GO :0045453 and bone resorption and biological process this GO :0050728 and negative regulation of inflammatory response and biological process this GO :0050830 and defense response to Gram-positive bacterium and biological process this GO :0060349 and bone morphogenesis and biological process this GO :0070062 and extracellular vesicular exosome and cellular component, this GO :0003993 : acid phosphatase activity, this GO :0003993 : acid phosphatase activity and also this GO :0008198 : ferrous iron binding and also this GO :0008199 : ferric iron binding and also this GO :0016787 : hydrolase activity, this GO :0008198 : ferrous iron binding, this GO :0008199 : ferric iron binding, this GO :0016787 : hydrolase activity, acid phosphatase 5 |
| Identity: |
124 |
| Gene: |
ACP5 |
More about : ACP5 |
| Long gene name: |
acid phosphatase 5, tartrate resistant |
| Synonyms: |
TRAP HPAP |
| Synonyms name: |
tartrate-resistant acid phosphatase human purple acid phosphatase |
| Locus: |
19p13, 2 |
| Discovery year: |
1989-02-23 |
| GenBank acession: |
X14618 |
| Entrez gene record: |
54 |
| Pubmed identfication: |
8449511 2338077 |
| Classification: |
Acid phosphatases |
| Havana BLAST/BLAT: |
OTTHUMG00000182036 |