| Antigen detectable with this ELISA kit: |
Ecto (NT5E), 5'-Nucleotidase |
| Species reactivity: |
Rattus norvegicus (Rat) |
| Range of detection: |
100ng/mL, 12, 25ng/mL, 3, 50ng/mL, 6, 125-200ng/mL The standard curve concentrations used for the ELISA&rsquo, 125ng/mL, 25ng/mL, 3, 5ng/mL, s were 200ng/mL |
| Sensitivity: |
27ng/mL, The minimum detectable dose of this kit is typically less than 1 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
plasma, tissue homogenates and other biological fluids, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Ecto (NT5E), Ecto (NT5E), Ecto (NT5E), Ecto (NT5E) in the samples is then determined by comparing the O, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 5'-Nucleotidase, The concentration of 5'-Nucleotidase, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to 5'-Nucleotidase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain 5'-Nucleotidase, D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Ecto (NT5E), 5' Nucleotidase |
| Short name: |
Ecto (NT5E) ELISA kit, 5'-Nucleotidase |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Ecto '-nucleotidase, ecto (CD73)) Enzyme-linked immunosorbent assay reagent, 5'-Nucleotidase |
| Alternative technique: |
elisas |
| Alternative to gene target: |
CALJA and CD73 and E5NT and eN and eNT and NT and NT5 and NTE, NT5E and IDBG-93979 and ENSG00000135318 and 4907, Nt5e and IDBG-190005 and ENSMUSG00000032420 and 23959, Plasma membranes, acting on ester bonds, acting on ester bonds and also this GO :0046872 : metal ion binding, acting on ester bonds and molecular function this GO :0031225 and anchored component of membrane and cellular component this GO :0044281 and small molecule metabolic process and biological process this GO :0046086 and adenosine biosynthetic process and biological process this GO :0046135 and pyrimidine nucleoside catabolic process and biological process this GO :0046872 and metal ion binding and molecular function this GO :0050728 and negative regulation of inflammatory response and biological process this GO :0055086 and nucleobase-containing small molecule metabolic process and biological process this GO :0070062 and extracellular vesicular exosome and cellular component, ecto (CD73), hydrolase activity, this GO :0000166 and nucleotide binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006144 and purine nucleobase metabolic process and biological process this GO :0006195 and purine nucleotide catabolic process and biological process this GO :0006196 and AMP catabolic process and biological process this GO :0006206 and pyrimidine nucleobase metabolic process and biological process this GO :0006259 and DNA metabolic process and biological process this GO :0008253 and 5'-nucleotidase activity and molecular function this GO :0009166 and nucleotide catabolic process and biological process this GO :0016020 and membrane and cellular component this GO :0016311 and dephosphorylation and biological process this GO :0016787 and hydrolase activity and molecular function this GO :0016788 and hydrolase activity, this GO :0000166 : nucleotide binding, this GO :0000166 : nucleotide binding and also this GO :0008253 : 5'-nucleotidase activity and also this GO :0016787 : hydrolase activity and also this GO :0016788 : hydrolase activity, this GO :0008253 : 5'-nucleotidase activity, this GO :0016787 : hydrolase activity, this GO :0016788 : hydrolase activity, this GO :0046872 : metal ion binding, 5'-nucleotidase |
| Identity: |
8021 |
| Gene: |
NT5E |
More about : NT5E |
| Long gene name: |
5', -nucleotidase ecto |
| Synonyms gene: |
NT5 |
| Synonyms gene name: |
5', ecto (CD73) , nucleotidase (CD73) 5', -nucleotidase |
| Synonyms: |
CD73 eN eNT CALJA |
| Locus: |
6q14, 3 |
| Discovery year: |
1989-02-23 |
| GenBank acession: |
X55740 |
| Entrez gene record: |
4907 |
| Classification: |
5', -nucleotidases CD molecules |
| Havana BLAST/BLAT: |
OTTHUMG00000015139 |