| Antigen detectable with this ELISA kit: |
Free (fT4), Thyroxine &ndash |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
The detection range is between 0-80 pg/ml |
| Sensitivity: |
0 pg/ml, The limit of detection is typically less than 1 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
Human Serum |
| Time for performing t hourse test: |
less t hoursan 2 hoursours |
| Test principle: |
A set of standards is used to plot a standard curve from which the amount of fT4 in patient samples and controls can be directly read, After the washing step, Competition occurs between an unlabeled antigen (present in standards, The absorbance is measured on a microtiter plate reader, The assay is carried out under normal physiological conditions of pH, The binding sites on the microwell plates are designed to be of a low binding-capacity in order not to disturb the equilibrium between T4 and its carrying proteins, The enzymatic reaction is terminated by addition of the stopping solution, The intensity of the colour formed is inversely proportional to the concentration of fT4 in the sample, The labeled T4 (conjugate) employed in this assay system has shown no binding properties towards thyroxine-binding globulin (TBG) and human serum albumin (HSA), The washing and decanting procedures remove unbound materials, controls and patient samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibody binding sites on the microwell plate, temperature and ionic strength, the enzyme substrate is added, The principle of the following enzyme immunoassay test follows the typical competitive binding scenario |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Free (fT4), Thyroxine &ndash |
| Short name: |
Free (fT4) ELISA kit, Thyroxine &ndash |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Free (fT4) Enzyme-linked immunosorbent assay reagent, Thyroxine &ndash |
| Alternative technique: |
elisas |