| Antigen detectable with this ELISA kit: |
Plasma Renin Activity |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
The detection range is between 0-60 ng/ml |
| Sensitivity: |
14 ng/ml, The limit of detection is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
Human Plasma |
| Time for performing t hourse test: |
less t hoursan 2 hoursours |
| Test principle: |
1987), A set of calibrators is used to plot a standard curve from which the concentrations of Ang-I in the samples and controls can be directly read, Adjustment of pH is a critical step during the assay, Before the start of immunoassay a protease inhibitor and the Generation buffer is added to the plasma sample, During the first incubation unlabelled Ang-I (present in the standards, During the immunoassay incubation, In the second incubation the labelled Streptavidin-HRP conjugate, Optionally, The absorbance values are inversely proportional to the concentration of Ang-I in the sample, The blood sample is collected in a tube that contains EDTA, The colorimetric HRP substrate is added and, The immunoassay of Ang-I is a competitive assay that uses two incubations, The pH of the plasma sample should be around 6, The plasma is separated and either stored frozen or kept at room temperature for immediate use, The plasma sample is split in two and the fractions are incubated at 0-4°, The washing and decanting procedures remove unbound materials, acidification of plasma to pH 3, after stopping the color development reaction, another set of protease inhibitors are involved, binds to the immobilized Ang-I-Biotin, controls and plasma samples) competes with biotinylated Ang-I to bind to the anti-Ang-I antibody, on the other side incubation at pH higher than 8, samples should not be chilled on ice or stored at temperatures between 0 and 10°, the light absorbance (OD) is measured in a microwell plate reader, the pH can be adjusted to 6, this could lead to overestimation of renin activity, to allow the generation of Ang-I by plasma renin at 37°, which function to stop the new generation as well as degradation of Ang-I to smaller peptides, which will prevent Angiotensin-I (Ang-I) in plasma from degradation, with a total assay incubation time of less than two hours, 0 after the addition of the supplied Generation buffer, 0 can destroy renin, 3 or lower for prolonged time with subsequent return to neutral pH causes irreversible activation of the renin (Derkx et al, 4, 5 or 7, C, C (in ice bath) and 37oC respectively for 90 minutes or longer, C during collection or processing before adjustment of pH, This kit measures PRA and the results are expressed in terms of mass of angiotensin-I (Ang-I) generated per volume of human plasma in unit time (ng/mL, h) |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Plasma Renin Activity |
| Short name: |
Plasma Renin Activity ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Plasma Renin Activity Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |