| Category: |
Antibody |
| Concentration: |
05% sodium azide, 1 mg/ml BSA (US sourced) and 0, 5 mg/ml in 1X PBS with 0 |
| Form: |
Purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
ICAD |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Mouse (Mus musculus) |
| Tested applications: |
WB |
| Recommended dilutions: |
Western blot: 4-8ug/ml |
| Notes: |
antibody titration may be required for optimal results, Provided assay concentrations are suggestions only |
| Added buffer: |
pH 7, 1X Phosphate Buffered Saline (PBS), 4 |
| Intented use: |
This ICAD antibodyis to be used only for research purposes and not for diagnostics |
| Gene ID #: |
1676 |
| Purity: |
Purified by protein A Affinity Chromatography |
| Description: |
Caspase-3 cleaves ICAD, DFF, DFFB is the active DFF subunit but remains inactive while bound to DFFA, The disassociation removes the inhibitory effects of ICAD, activating DFFB and beginning the DNA fragmentation process, also call ICAD (Inhibitor of caspase-activated DNase), is a heterodimeric protein which mediates DNA fragmentation and chromatin condensation during apoptosis, or DNA fragmentation factor, toxic or useless cells are broken down for removal, which then disassociates from DFFB, During apoptosis |
| Immunogen: |
A recombinant protein fragment from human ICAD was used as the immunogen for this antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
ICAD |
| Short name: |
ICAD Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
ICAD (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
2772 |
| Gene: |
DFFA |
More about : DFFA |
| Long gene name: |
DNA fragmentation factor subunit alpha |
| Synonyms gene name: |
45 kD, 45kDa, DNA fragmentation factor, alpha polypeptide , alpha polypeptide DNA fragmentation factor |
| Synonyms: |
DFF-45 DFF45 ICAD DFF1 |
| Synonyms name: |
45 kD, DNA fragmentation factor, alpha subunit |
| Locus: |
1p36, 22 |
| Discovery year: |
1997-10-27 |
| GenBank acession: |
AF087573 |
| Entrez gene record: |
1676 |
| Pubmed identfication: |
9605855 9108473 |
| RefSeq identity: |
NM_004401 |
| Havana BLAST/BLAT: |
OTTHUMG00000001909 |