| known also as: |
conjugated with Biotin, Anti-Rabbit anti pigeon IgG (heavy and light chains) |
| known also as: |
conjugated with Biotin, Rabbit anti pigeon IgG (heavy and light chains) |
| known also as: |
conjugated with Biotin, Lapin anti pigeon IgG (heavy and light chains) |
| known also as: |
Rabbit anti pigeon IgG (heavy and light chains) |
| Category: |
Secondary Antibodies |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Host organism: |
Rabbit |
| Species reactivity: |
The degree of cross-reactivity depends in addition to the phylogenetic relationship also on the con, since Ig of different species frequently share antigenic determinants, Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins |
| Specificity and cross-reactivity: |
II does not react with any non-Ig protein in pigeon serum, It includes a certain degree of reactivity with other immunoglobulins via the common F ab portion, as tested by immunoelectrophoresis and double radial immuno-diffusion, he reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule, heavy and light chains, Biotin-conjugated IgG fraction of polyclonal Rabbit antiSerum to pigeon IgG |
| Purification method: |
Hyperimmune antisera with sirong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromalography |
| Form/Appearance: |
pH 7, 2), Biotin-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS |
| Storage and shipping: |
= at least 10 years reconstituted at or below Store productone at -20 degrees Celsius, =3-5 years reconstituted at +Store productone at +4 degrees Celsius, For in vitro laboratory research purposes only, If a slight precipitation occurs upon storage, It is reconstituted by adding 1 ml sterile distilled water, It will not affect the performance of the immunoconjugate, Prior to use, Repeated thawing and freezing should be avoided, Storage I shelf life: lyophilized at +Store productone at +4 degrees Celsius, Working dilutions should be stored at +Store productone at +4 degrees Celsius, an aliquot is thawed slowly in the dark at ambient temperature, and of all associated materials, and preferably used the same day, divided into small aliquots, frozen and stored at or below Store productone at -20 degrees Celsius, not refrozen, pH 7, prolonged storage at or below Store productone at -20 degrees Celsius, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, spun down to remove insoluble particles, this should be removed by centrifugation, 2), =7 days Caution: This immunoconjugate should be handled by qualified persons only and appropriate precautions should be taken in its handling and disposal, Handling: The lyophilized conjugate is shipped at ambient temperature and may be stored at +Store productone at +4 degrees Celsius |
| Tested for:: |
Dot Blot (DB), Immunoblotting (IB), Immunocytochemistry (IHC), Immunohistochemistry (IHC), ELISA (EIA) |
| Description: |
Polyclonal antibodies generally are more stable and retain their reactivity under unfavorable conditions, Polyclonal antibodies have series of advantages - larger batches can be supplied at a time, Properly used, To obtain more detailed information on productone, please, refer to the full product datasheet, the time needed for production is considerably shorter, they are inexpensive to manufacture and respectively to buy, this antibody will ensure excellent and reproducible results with guaranteed success for the applications that it is tested in, productone is a polyclonal antibody of high purity and binding affinity for the antigen that it is risen against |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, avoid cycles of freezing and thawing, please, In order to retain the quality and the affinity of productone unchanged |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, Biotin conjugates can be detected by horseradish peroxidase |
| Conjugation: |
Biotinylated |
| About: |
2 light chains, For storage sodium azide is added or you can call us to request azide free antibody preparations, IgG, The IgG antibody has 2 , There are 2 heavy chains, These will need colder storage temperatures, They represent 70% or more of , This antibody can be antigen purified or protein A or G purified, goat polyclonal antibodies , mouse monoclonal H&, Immunoglobulin gamma, L chain clones or rabbit, antibodies, antigen , binding sites, have 4 parts, serum |
| Gene target: |
(heavy light chains) conjugated with |
| Short name: |
Anti-pigeon IgG (heavy light chains) conjugated with |
| Technique: |
IgGs, light |
| Isotype: |
IgG |
| Label: |
Biotin |
| Alternative name: |
coupled including biotinilated, antibody to-pigeon Immunoglobulin G (heavy and light chains) |
| Alternative technique: |
immunoglobulins |