| known also as: |
conjugated with FITC, Anti-Goat anti Chicken IgM (Fc specific) |
| known also as: |
conjugated with FITC, Caprine anti Chicken IgM (Fc specific) |
| known also as: |
conjugated with FITC, Caprinae anti Gallus gallus IgM (Fc specific) |
| known also as: |
Goat anti Chicken IgM (Fc specific) |
| Category: |
Secondary Antibodies |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Host organism: |
Goat |
| Species reactivity: |
Cross-reactivity of this antiserum has not been tested in detail, This immunoconJugate is not species-specific since Inter-species cross-reactivity is a normal feature of antisera to immunoglobulins |
| Specificity and cross-reactivity: |
IgG/Fab fragments and IgA or any non-Ig protein in chicken serum, In immunoelectrophoresis and radial in immunodiffusion using various antiserum concentrations against chicken serum, It does not react with IgG, a single precipitin line has been obtained which shows a reaction of identity with the precipitin lines obtained with the purified IgM used as Immunogens, as tested by Immunoelectrophoresis and double radial immunodiffusion, which expresses strict (class) specificity, The reactivity of the antiserum IS directed to the Fc subunit of the IgM molecule |
| Purification method: |
It IS free of other serum proteins as tested by immunoelectrophoresis, Hyperimmune antisera with strong precipitating activity are selected for fractionation and purification of the IgG (7S) fraction containing the bulk of the defined antibody specificity |
| Form/Appearance: |
pH 7, 2), FITC-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS |
| Storage and shipping: |
If a slight precipitation occurs upon storage, It is reconstituted by adding 1 ml sterile distilled water, It will not affect the performance of the immunoconjugate, Packing: Vial with 1 mllyophilized immunoconjugate, Prior to use, Repeated thawing and freezing should be avoided, Storage and Shelf life: Lyophilized at +4'C = at least 10 years reconstituted at or below -20'C =3-5 years reconstituted at +4 'C =7 days_x000D_ _x000D_ _x000D_, Working dilutions should be stored at +4'C, an aliquot is thawed slowly at ambient temperature, and preferably used the same day, divided into small aliquots, frozen and stored at or below -20'C, not refrozen, pH 7, prolonged storage at or below -20'C, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, spun down to remove insoluble particles, this should be removed by centrifugation, 2), Handling: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4'C |
| Tested for:: |
(In)direct Immunofluorescence (IF), Immunocytochemistry (IHC), Immunohistochemistry (IHC), ELISA (EIA) |
| Description: |
Freeze thaw will destroy a percentage in every cycle and should be avoided, C in a fridge short term in a concentrated dilution, This antibody needs to be stored at + 4° |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, avoid cycles of freezing and thawing, please, In order to retain the quality and the affinity of productone unchanged |
| Properties: |
Chickens like all bird species have IgY antibodies that are very stable and found in the yolk, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, Gallus Gallus domestica is the Latin name of the Chicken, IgM is by far the physically largest antibody in the human circulatory system, Immunoglobulin M, It is the first antibody to appear in response to initial exposure to an antigen, MBS Polyclonals_1 FITC is highly sensitive to pH extremes, This MBS Polyclonals_1 Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, is a basic , its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, or , the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, B cells, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by MBS Polyclonals_1 they should be stored frozen at - 24°, IgM , antibody , for short, that is produced by |
| Conjugation: |
Anti-FITC Antibody |
| Latin name: |
Capra aegagrus hircus |
| Gene target: |
(Fc specific) conjugated with |
| Short name: |
Goat anti Chicken IgM (Fc specific) conjugated with FITC Antibody |
| Technique: |
Fluorescein, IgMs, antibodies against human proteins, antibodies for, antibody to, goats, Antibody |
| Host: |
It are very good secondary antibodies that are affinity purified and have a good affinity themselves too, Often KLH is coupled to the antigen so anti KLH can be present in the vial, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection, Goat |
| Isotype: |
IgM |
| Label: |
FITC |
| Species: |
Chickens, Goats, Chicken |
| Alternative name: |
coupled including fluorecein (Antibody to), caprine antibody to Chicken Immunoglobulin M (fragment c specific) |
| Alternative technique: |
antibodies |