| We send this reagant on: |
Blue Ice |
| The temperatureerature for storage is: |
Aliquot as needed to avoid repeated freeze-thaw cycles and store at -20°, If precipitates or turbidity are observed upon thawing, Store the Triton X-100 Solution at Room temperatureerature, ready-to-use solution, slightly red, warm the solution to 37°, C for 5-10 minutes and agitate to dissolve the precipitates, C protected from light, The LDH Cytotoxicity Assay Reagent is a clear |
| SDS or safty data sheet: |
more (CBA-241) GHS, pdf |
| Type of reagent: |
Cell Based Assays |
| Related techniques: |
Cell Health Assays >, LDH Cytotoxicity Assay, Cell Based Assays, Cytotoxicity Assays |
| Use of the reagent: |
, An increase in cell cytotoxicity is accompanied by increased LDH release and increased colorimetry signal, Cells can be plated and then treated with compounds or agents that affect cell viability, CytoSelect&trade, LDH Cytotoxicity Assay test kit provides a colorimetry format for measuring and monitoring cell cytotoxicity, The LDH Cytotoxicity Reagent can be used to detect cytotoxicity in bacteria, The WST-1 molecule, The assay principles are basic and can be applied to most eukaryotic cell lines, The growth media is then transferred to another plate and the released LDH is then detected with cytotoxicity reagent, The test kit contains sufficient reagents for the evaluation of 960 assays in 96-well plates, Upon cell death, a soluble enzyme found in the cytoplasm, also present in the LDH Cytotoxicity Reagent, depending on LDH expression levels, fungi, including adherent and non-adherent cells and certain tissues, is converted from WST-1 to the orange formazan form, is released into the growth media, lactate dehydrogenase (LDH), protozoa as well as cultured mammalian and piscine cells, yeast, , , , , , , , , , Cell Biolabs&rsquo, In the presence of lactate substrate (included in the LDH Cytotoxicity Reagent) LDH converts lactate to pyruvate and generates nicotinamide adenine dinucleotide (NADH) |
| Cellular use: |
fungi, protozoa as well as cultured mammalian and piscine cells, yeast, LDH Cytotoxicity Reagent can be used to detect cytotoxicity in bacteria |
| Protocol information: |
Cells can be plated and then treated with compounds or agents that affect cell viability |
| Reagent properties: |
containing sufficient reagents for the evaluation of 960 assays in 96-well plates, colorimetry , test kit |
| Living modified organisme in this reagent or LMO: |
Not applicable |
| Conjugation and form: |
host specific |
| Species compatible to: |
human cells and cell lines and other cells |
| Use of this test kit or product: |
Molecular biology |
| Antigen: |
the sequence of the protein |
| Buffer: |
45, The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7 |
| Molecular weight: |
kiloDalton |
| This reagent is purified: |
98 |
| Test: |
Cellular biology labs supplies other types of Assays as 1 |
| Gene target: |
LDH Cytotoxicity test kit, CytoSelect&trade |
| Short name: |
LDH Cytotoxicity Assay test kit, CytoSelect&trade |
| Technique: |
assays, Assay |
| Host: |
Assay |
| Alternative name: |
LDH Cytotoxicity test test reagent, CytoSelect&trade |
| Alternative technique: |
arrays |