| Long name: |
1) Monoclonal Antibody, 2, 3, ESR1/ER (415CT16 |
| Also known as: |
1) Monoclonal Antibody, 2, 3, ESR1/ER (415CT16 |
| Category: |
Primary Antibodies |
| Conjugation: |
Unconjugated |
| Target Antigen: |
ESR1/ER |
| Specificity: |
This is a highly specific antibody against ESR1/ER |
| Modification(s): |
None |
| Modification site(s): |
Unmodified antibody |
| Clonality: |
Monoclonal |
| Clone number: |
1, 2, 3, 415CT16 |
| Source: |
This ESR1/ER antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 246-273 amino acids from human ESR1/ER |
| Gene ID number: |
2099 |
| Swiss Prot: |
P03372 |
| Tested Applications: |
WB |
| Recommended dilutions: |
WB(1:100~250) |
| Cross reactivity: |
Human |
| Cross reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the target antigen: |
ATF-2, Also mediates membrane-initiated estrogen signaling involving various kinase cascades, Can activate the transcriptional activity of TFF1, Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements, Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter, Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3, Isoform 3 can bind to ERE and inhibit isoform 1, Isoform 3 is involved in activation of NOS3 and endothelial nitric oxide production, Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full length receptor, Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components, Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner, Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences, Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP, Sp1 and Sp3, The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues, c-Fos, such as AP-1/c-Jun, the function involves CREBBP, to mediate ERE-independent signaling, Nuclear hormone receptor |
| Purification method: |
This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS, 09% (W/V) sodium azide, Purified monoclonal antibody supplied in PBS with 0 |
| Storage conditions: |
50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Synonym names: |
ER-alpha, ESR, ESR1, ESRA, ESTRR, Era, Estradiol receptor, Estrogen receptor, NR3A1, Nuclear receptor subfamily 3 group A member 1, ER |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24° |
| About: |
Each murine monoclonal anibody has his own affinity specific for the clone, Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes, Monoclonals of this antigen are available in different clones |
| Gene target: |
1) Monoclonal, 2, 3, ESR1/ER (415CT16 |
| Short name: |
1) Monoclonal Antibody, 2, 3, ESR1/ER (415CT16 |
| Technique: |
Monoclonals or monoclonal antibodies, antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
1) monoclonal (Antibody to), 2, 3, ESR1/ER (415CT16 |
| Alternative technique: |
antibodies |
| Identity: |
3467 |
| Gene: |
ESR1 |
More about : ESR1 |
| Long gene name: |
estrogen receptor 1 |
| Synonyms gene: |
ESR |
| Synonyms: |
NR3A1 Era |
| Locus: |
6q25, 1-q25, 2 |
| Discovery year: |
2001-06-22 |
| GenBank acession: |
X03635 |
| Entrez gene record: |
2099 |
| Pubmed identfication: |
3754034 |
| Classification: |
Nuclear hormone receptors |
| Havana BLAST/BLAT: |
OTTHUMG00000016103 |
| Locus Specific Databases: |
LRG_992 |