| Also known as: |
Histone H1 |
| Other names: |
N/A, N/A, N/A, histone H1, histone H1 |
| Category: |
Antibodies |
| Subcategory: |
Mnoclonal antibodies |
| Gene name: |
N/A, N/A, leH1, H1 |
| Gene name synonims: |
N/A, N/A, leH1, H1 |
| Other gene names: |
N/A, N/A, leH1, H1 |
| Clonality: |
Monoclonal |
| Immunoglobulin isotype: |
IgG2a |
| Clone: |
AE-4 |
| Host organism: |
Mouse (Mus musculus) |
| Species reactivity: |
Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, Human (Homo sapiens) |
| Specificity and cross-reactivity: |
H2A, H2B, H3 and H4 that assemble to form an octamer round which the DNA is wrapped in a sequence dependant manner, Histone H1 acts as a linker and binds to each nucleosome, In blots of rat brain homogenate a duplet is observed at 27-29 kD consistent with Histone H1, In eukaryotic cells there are 4 core histone proteins, Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, Speckled staining is observed in the nucleus of normal and malignant cells, The histone proteins serve as a structural scaffold for the organization of nuclear DNA into chromatin, The resulting complex is known as a nucleosome and is the basic subunit of chromatin, This particular antibody might cross react with speacies outside of the listed ones, and by self affinity, links these nucleosomes together resulting in the 30 nm chromatin fiber, Recognizes histone H1 |
| Purification method: |
Protein G Purified |
| Form/Appearance: |
0, 09% sodium azide, PBS |
| Concentration: |
1 miligram per 1 mililiter |
| Storage and shipping: |
Cycles of freezing and defrosting may denaturate the polypeptide chains of the antibodies and reduce their activity, For longer storage the antibody should be kept at -20 degrees Celsius, For short term storage keep the antibody at +4 degrees Celsius |
| Tested applications:: |
ELISA (EIA), IHC - Frozen), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC - Paraffin |
| Properties: |
Depending on the epitopes used human ELISA kits can be cross reactive to many other species, Human proteins, Mainly analyzed are human serum, Modern , cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies, human cell culture supernatants and biological samples, plasma, primarily , saliva, urine, , (Homo sapiens, C, C for long term storage and for short term at + 5°, Homo sapiens sapiens), If you buy Antibodies supplied by MBS mono they should be stored frozen at - 24°, humans , ssp |
| About: |
Each murine monoclonal anibody has his own affinity specific for the clone, Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes, Monoclonals of this antigen are available in different clones |
| Test: |
A/J, BALB/c, Mouse are mature after 40 days for females and 55 days for males, SCID while the CD-1 is outbred as strain, The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year, Transgenic, congenic and inbread strains are known for C57BL/6, knock-out, Mouse , mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab, or |
| Latin name: |
Mus musculus |
| Gene target: |
Monoclonal [ AE-4] (IgG2a) Histone H1 |
| Short name: |
Mouse Monoclonal [clone AE-4] (IgG2a) Histone H1 Antibody |
| Technique: |
Monoclonals or monoclonal antibodies, antibodies against human proteins, antibodies for, mouses, Antibody |
| Isotype: |
IgG2a |
| Species: |
Humans, Mouses, Human |
| Alternative name: |
sapiens Histone H1 (Antibody to), Mouse monoclonal [clonality AE-4] (IgG2a) to H |
| Alternative technique: |
antibodies |