| Also known as: |
SIGLEC-10 |
| Other names: |
Sialic acid-binding Ig-like lectin 10, Siglec-like protein 2, sialic acid binding Ig-like lectin 10, sialic acid binding Ig-like lectin 10 Ig-like lectin 7, sialic acid-binding Ig-like lectin 10, siglec-like gene 2, siglec-like protein 2, sialic acid-binding Ig-like lectin 10 isoform 2 |
| Category: |
Antibodies |
| Subcategory: |
Mnoclonal antibodies |
| Gene name: |
SIGLEC-10 |
| Other gene names: |
PRO940, SIGLEC-10, SIGLEC10, SLG2, SLG2, Siglec-10, SIGLEC10 |
| Clonality: |
Monoclonal |
| Immunoglobulin isotype: |
IgG1 |
| Clone: |
5G6 |
| Host organism: |
Mouse (Mus musculus) |
| Species reactivity: |
Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, Human (Homo sapiens) |
| Specificity and cross-reactivity: |
5, 7, 8 and 9_x000D__x000D_, B cells, Siglec-10 is most similar to the CD33-related group of Siglecs, Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, Studies have shown that Siglec-10 acts as a substrate for VAP-1 (Vascular adhesion protein-1), This particular antibody might cross react with speacies outside of the listed ones, _x000D__x000D_Structurally, _x000D__x000D_Studies have shown that clone 5G6 does not cross-react with Siglecs 3, a glycoprotein expressed on endothelium during inflammation, a putative adhesion molecule and member of the Ig superfamily, and at a higher level by a subpopulation of CD16<, and preferentially binds to glycoconjugates containing alpha-2, eosinophils, expressed by monocytes, implicates Siglec-10 in endothelial lymphocyte adhesion and in the modulation of the inflammatory microenvironment, natural killer (NK) cells, which is involved in primary amine oxidation and leucocyte trafficking This interaction between Siglec-10 and VAP-1, +<, -<, /sup>, /sup>, 3- or alpha-2, 6-linked sialic acid, CD56<, SIGLEC-10 Clone 5G6 specifically recognises human Siglec-10 (Sialic acid-binding Ig-like lectin 10), sup>, sup> |
| Form/Appearance: |
FITC (Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid) |
| Concentration: |
1 miligram per 1 mililiter, IgG concentration 0 |
| Storage and shipping: |
If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use, Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided, Shelf Life: 18 months from date of dispatch, the antibody is photosensitive and should be protected from light, the antibody should be stored undiluted, Storage in frost-free freezers is not recommended, Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term |
| Tested applications:: |
Flow Cytometry (FACS) |
| Properties: |
FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, MBS mono FITC is highly sensitive to pH extremes, This MBS mono Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by MBS mono they should be stored frozen at - 24° |
| Conjugation: |
Anti-FITC Antibody |
| Gene target: |
SIGLEC-10: |
| Short name: |
MOUSE Anti-HUMAN SIGLEC-10:FITC Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, Antibody |
| Label: |
FITC |
| Alternative name: |
sapiens SIGLEC-10:fluorecein (Antibody to), MOUSE antibody to-H |
| Alternative technique: |
antibodies |
| Identity: |
15620 |
| Gene: |
SIGLEC10 |
More about : SIGLEC10 |
| Long gene name: |
sialic acid binding Ig like lectin 10 |
| Synonyms gene name: |
sialic acid binding Ig-like lectin 10 |
| Synonyms: |
SIGLEC-10 SLG2 PRO940 MGC126774 |
| Synonyms name: |
sialic acid binding Ig-like lectin 10 Ig-like lectin 7 siglec-like gene 2 |
| Locus: |
19q13, 41 |
| Discovery year: |
2001-06-29 |
| GenBank acession: |
AF310233 |
| Entrez gene record: |
89790 |
| Pubmed identfication: |
11284738 |
| RefSeq identity: |
NM_033130 |
| Classification: |
C2-set domain containing Sialic acid binding Ig like lectins V-set domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000165521 |