| Long name: |
Biotin Conjugated, ZWILCH Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
ZWILCH |
| Specificity: |
This is a highly specific antibody against ZWILCH |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human ZWILCH |
| Gene ID number: |
55055 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Mutations were discovered in Zwilch, The Drosophila Zwilch forms a complex with both ROD Rough Deal) and ZWINT (Zeste-White 10, The human Zwilch, The role of Zwilch in complex is thought to be evolutionarily conserved because the human homologs of Zwilch, These mutations may contribute in part to the chromosomal instability phenotype of colorectal tumor cells, This complex is important for chromosome segregation because it recruits cytoplasmic Dynein to the kinetochore and plays a crucial role in the spindle checkpoint, ZWINT and ROD coimmunoprecipitate in a human cell line called HeLa, ZWINT and ROD complex localizes to the kinetochores at prometaphase, ZWINT and ROD during a screen for mutations in alleles encoding putative chromosome instability genes in cases of human colorectal cancer, also designated ZW10) proteins, Zwilch is the human homolog of the Drosophila Zwilch protein |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
FLJ10036 KNTC1AP |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
ZWILCH Conjugated |
| Short name: |
Conjugated, ZWILCH Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, homolog (Drosophila) (Antibody to), kinetochore associated, Zwilch |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
Cytoplasm, ZWILCH and IDBG-18211 and ENSG00000174442 and 55055, ZWILCH and IDBG-645460 and ENSBTAG00000005213 and 534811, Zwilch and IDBG-175909 and ENSMUSG00000032400 and 68014, homolog (Drosophila), kinetochore associated, protein binding, this GO :0000278 and mitotic cell cycle and biological process this GO :0000776 and kinetochore and cellular component this GO :0000777 and condensed chromosome kinetochore and cellular component this GO :0005515 and protein binding and molecular function this GO :0005829 and cytosol and cellular component this GO :0007067 and mitotic nuclear division and biological process this GO :0007093 and mitotic cell cycle checkpoint and biological process, this GO :0005515 : protein binding, this GO :0005515 : protein binding, Zwilch |
| Identity: |
25468 |
| Gene: |
ZWILCH |
More about : ZWILCH |
| Long gene name: |
zwilch kinetochore protein |
| Synonyms gene name: |
Zwilch, homolog (Drosophila) , kinetochore associated |
| Locus: |
15q22, 31 |
| Discovery year: |
2005-07-25 |
| GenBank acession: |
AK023175 |
| Entrez gene record: |
55055 |
| Pubmed identfication: |
12686595 |
| RefSeq identity: |
NM_017975 |
| Havana BLAST/BLAT: |
OTTHUMG00000133194 |