| Long name: |
FITC Conjugated, RNF75 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
RNF75 |
| Specificity: |
This is a highly specific antibody against RNF75 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human ROC1/RNF75 |
| Gene ID number: |
9978 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
As a component of the CSA complex promotes the ubiquitination of ERCC6 resulting in proteasomal degradation, CUL2, CUL4 and CUL4 via its interaction with UBE2M, May be required for histone H3 and histone H4 ubiquitination in response to ultraviolet and for subsequent DNA repair, Probably also stimulates CDC34 autoubiquitination, Promotes the neddylation of CUL1, The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate recognition components, Through the RING-type zinc finger, including proteins involved in cell cycle progression, like CDC34, seems to recruit the E2 ubiquitination enzyme, signal transduction, to the complex and brings it into close proximity to the substrate, transcription and transcription-coupled nucleotide excision repair, E3 ubiquitin ligase component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
ROC1 RNF75 BA554C12, 1 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
RNF75 Conjugated |
| Short name: |
FITC Conjugated, RNF75 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, RNF75 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
9928 |
| Gene: |
RBX1 |
More about : RBX1 |
| Long gene name: |
ring-box 1 |
| Synonyms gene name: |
E3 ubiquitin protein ligase , ring-box 1 ring-box 1 |
| Synonyms name: |
regulator of cullins 1 |
| Locus: |
22q13, 2 |
| Discovery year: |
1999-10-29 |
| GenBank acession: |
AF140598 |
| Entrez gene record: |
9978 |
| Pubmed identfication: |
10213691 10230407 |
| RefSeq identity: |
NM_014248 |
| Classification: |
Ring finger proteins SCF complex |
| Havana BLAST/BLAT: |
OTTHUMG00000151298 |