| Long name: |
FITC Conjugated, LRDD Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
LRDD |
| Specificity: |
This is a highly specific antibody against LRDD |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human LRDD/PIDD |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Increased PIDD expression results in spontaneous activation of caspase-2 and sensitization to apoptosis by genotoxic stimuli, Independently, PIDD also promotes apoptosis downstream of p53 as component of the DNA damage/stress response pathway that connects p53/TP53 to apoptosis, PIDD forms a complex with caspase-2 and the adaptor protein RAIDD, PIDD has also been shown to interact with NEMO/IKBKG and RIP1 and enhance sumoylation and ubiquitination of NEMO/IKBKG, PIDD was also described as a DD-containing protein with unknown function, The N-terminal region of PIDD contains seven leucine-rich repeats (LRRs), a protein interaction motif found in various proteins with diverse functions, an important component for activation of the transcription factor NF-kappa-B, followed by two ZU-5 domains and a C-terminal DD, via interaction with caspase-2 and CRADD/RAIDD, The death domain (DD) containing protein PIDD is a p53 target gene in an erythroleukemia cell line that undergoes G1 phase arrest and subsequent apoptosis after p53 expression |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
MGC16925 DKFZp434D229 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
LRDD Conjugated |
| Short name: |
FITC Conjugated, LRDD Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, LRDD (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
16491 |
| Gene: |
PIDD1 |
More about : PIDD1 |
| Long gene name: |
p53-induced death domain protein 1 |
| Synonyms gene: |
LRDD PIDD |
| Synonyms gene name: |
leucine-rich repeats and death domain containing p53-induced death domain protein |
| Locus: |
11p15, 5 |
| Discovery year: |
2001-09-04 |
| GenBank acession: |
AF229178 |
| Entrez gene record: |
55367 |
| Pubmed identfication: |
10825539 |
| RefSeq identity: |
NM_145886 |
| Havana BLAST/BLAT: |
OTTHUMG00000133306 |