| Long name: |
FITC Conjugated, HRAS+KRAS Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
HRAS+KRAS |
| Specificity: |
This is a highly specific antibody against HRAS+KRAS |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human HRAS+KRAS |
| Gene ID number: |
3265 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
HRAS, The RAS proteins are GDP/GTP-binding proteins that act as intracellular signal transducers, The most well-studied members of the RAS (derived from 'RAt Sarcoma' virus) gene family include KRAS, These genes encode immunologically related proteins with a molecular mass of 21 kD and are homologs of rodent sarcoma virus genes that have transforming abilities, While these wildtype cellular proteins in humans play a vital role in normal tissue signaling, and NRAS, and senescence, differentiation, including proliferation, mutated genes are potent oncogenes that play a role in many human cancers, The KRAS gene encodes the human cellular homolog of a transforming gene isolated from the Kirsten rat sarcoma virus |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
HRAS+KRAS Conjugated |
| Short name: |
FITC Conjugated, HRAS+KRAS Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, v-Ha-ras Harvey rat sarcoma viral oncogene homolog+v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (Antibody to) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
C-BAS/HAS and C-H-RAS and C-HA-RAS1 and CTLO and H-RASIDX and HAMSV and HRAS1 and p21ras and RASH1, C-K-RAS and CFC2 and K-RAS2A and K-RAS2B and K-RAS4A and K-RAS4B and KI-RAS and KRAS1 and KRAS2 and NS and NS3 and RASK2, GABAergic and biological process this GO :0032403 and protein complex binding and molecular function this GO :0035022 and positive regulation of Rac protein signal transduction and biological process this GO :0035176 and social behavior and biological process this GO :0038095 and Fc-epsilon receptor signaling pathway and biological process this GO :0043406 and positive regulation of MAP kinase activity and biological process this GO :0043524 and negative regulation of neuron apoptotic process and biological process this GO :0045087 and innate immune response and biological process this GO :0045121 and membrane raft and cellular component this GO :0045596 and negative regulation of cell differentiation and biological process this GO :0048011 and neurotrophin TRK receptor signaling pathway and biological process this GO :0048169 and regulation of long-term neuronal synaptic plasticity and biological process this GO :0050900 and leukocyte migration and biological process this GO :0051000 and positive regulation of nitric-oxide synthase activity and biological process this GO :0051092 and positive regulation of NF-kappaB transcription factor activity and biological process this GO :0051146 and striated muscle cell differentiation and biological process this GO :0051384 and response to glucocorticoid and biological process this GO :0051385 and response to mineralocorticoid and biological process this GO :0060441 and epithelial tube branching involved in lung morphogenesis and biological process, GABAergic and biological process this GO :0032855 and positive regulation of Rac GTPase activity and biological process this GO :0034259 and negative regulation of Rho GTPase activity and biological process this GO :0035022 and positive regulation of Rac protein signal transduction and biological process this GO :0035176 and social behavior and biological process this GO :0038095 and Fc-epsilon receptor signaling pathway and biological process this GO :0043231 and intracellular membrane-bounded organelle and cellular component this GO :0043406 and positive regulation of MAP kinase activity and biological process this GO :0043410 and positive regulation of MAPK cascade and biological process this GO :0043524 and negative regulation of neuron apoptotic process and biological process this GO :0045087 and innate immune response and biological process this GO :0045596 and negative regulation of cell differentiation and biological process this GO :0045740 and positive regulation of DNA replication and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0046330 and positive regulation of JNK cascade and biological process this GO :0046579 and positive regulation of Ras protein signal transduction and biological process this GO :0048011 and neurotrophin TRK receptor signaling pathway and biological process this GO :0048169 and regulation of long-term neuronal synaptic plasticity and biological process this GO :0048471 and perinuclear region of cytoplasm and cellular component this GO :0050679 and positive regulation of epithelial cell proliferation and biological process this GO :0050900 and leukocyte migration and biological process this GO :0051146 and striated muscle cell differentiation and biological process this GO :0051291 and protein heterooli this GO merization and biological process this GO :0060441 and epithelial tube branching involved in lung morphogenesis and biological process this GO :0070374 and positive regulation of ERK1 and ERK2 cascade and biological process this GO :0090303 and positive regulation of wound healing and biological process this GO :0090398 and cellular senescence and biological process this GO :0097193 and intrinsic apoptotic signaling pathway and biological process this GO :1900029 and positive regulation of ruffle assembly and biological process this GO :2000251 and positive regulation of actin cytoskeleton reorganization and biological process this GO :2000630 and positive regulation of miRNA metabolic process and biological process, HRAS and IDBG-16878 and ENSG00000174775 and 3265, HRAS and IDBG-645593 and ENSBTAG00000046644 and 100298939, Hras1 and IDBG-212024 and ENSMUSG00000025499 and 15461, KRAS and IDBG-23889 and ENSG00000133703 and 3845, KRAS and IDBG-638783 and ENSBTAG00000009778 and 541140, Kras and IDBG-197719 and ENSMUSG00000030265 and 16653, Plasma membranes, ferrous iron transmembrane transporter activity, nuclei, protein complex binding, this GO :0000139 and this GO lgi membrane and cellular component this GO :0000165 and MAPK cascade and biological process this GO :0000186 and activation of MAPKK activity and biological process this GO :0001934 and positive regulation of protein phosphorylation and biological process this GO :0003924 and GTPase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005525 and GTP binding and molecular function this GO :0005622 and intracellular and cellular component this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005794 and this GO lgi apparatus and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006184 and GTP catabolic process and biological process this GO :0006897 and endocytosis and biological process this GO :0006915 and apoptotic process and biological process this GO :0006935 and chemotaxis and biological process this GO :0007050 and cell cycle arrest and biological process this GO :0007093 and mitotic cell cycle checkpoint and biological process this GO :0007165 and signal transduction and biological process this GO :0007166 and cell surface receptor signaling pathway and biological process this GO :0007173 and epidermal growth factor receptor signaling pathway and biological process this GO :0007264 and small GTPase mediated signal transduction and biological process this GO :0007265 and Ras protein signal transduction and biological process this GO :0007268 and synaptic transmission and biological process this GO :0007411 and axon guidance and biological process this GO :0007569 and cell aging and biological process this GO :0007596 and blood coagulation and biological process this GO :0008022 and protein C-terminus binding and molecular function this GO :0008283 and cell proliferation and biological process this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0008286 and insulin receptor signaling pathway and biological process this GO :0008542 and visual learning and biological process this GO :0008543 and fibroblast growth factor receptor signaling pathway and biological process this GO :0009887 and organ morphogenesis and biological process this GO :0010628 and positive regulation of gene expression and biological process this GO :0010629 and negative regulation of gene expression and biological process this GO :0015031 and protein transport and biological process this GO :0015093 and ferrous iron transmembrane transporter activity and molecular function this GO :0015684 and ferrous iron transport and biological process this GO :0016020 and membrane and cellular component this GO :0016021 and integral component of membrane and cellular component this GO :0030036 and actin cytoskeleton organization and biological process this GO :0030335 and positive regulation of cell migration and biological process this GO :0032228 and regulation of synaptic transmission, this GO :0000165 and MAPK cascade and biological process this GO :0000186 and activation of MAPKK activity and biological process this GO :0001934 and positive regulation of protein phosphorylation and biological process this GO :0003924 and GTPase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005525 and GTP binding and molecular function this GO :0005622 and intracellular and cellular component this GO :0005739 and mitochondrion and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006184 and GTP catabolic process and biological process this GO :0006886 and intracellular protein transport and biological process this GO :0006913 and nucleocytoplasmic transport and biological process this GO :0007165 and signal transduction and biological process this GO :0007173 and epidermal growth factor receptor signaling pathway and biological process this GO :0007264 and small GTPase mediated signal transduction and biological process this GO :0007265 and Ras protein signal transduction and biological process this GO :0007411 and axon guidance and biological process this GO :0007596 and blood coagulation and biological process this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0008286 and insulin receptor signaling pathway and biological process this GO :0008542 and visual learning and biological process this GO :0008543 and fibroblast growth factor receptor signaling pathway and biological process this GO :0010628 and positive regulation of gene expression and biological process this GO :0015031 and protein transport and biological process this GO :0016020 and membrane and cellular component this GO :0019002 and GMP binding and molecular function this GO :0019003 and GDP binding and molecular function this GO :0019221 and cytokine-mediated signaling pathway and biological process this GO :0030036 and actin cytoskeleton organization and biological process this GO :0030275 and LRR domain binding and molecular function this GO :0032228 and regulation of synaptic transmission, this GO :0003924 : GTPase activity, this GO :0003924 : GTPase activity, this GO :0003924 : GTPase activity and also this GO :0005515 : protein binding and also this GO :0005525 : GTP binding and also this GO :0008022 : protein C-terminus binding and also this GO :0015093 : ferrous iron transmembrane transporter activity, this GO :0003924 : GTPase activity and also this GO :0005515 : protein binding and also this GO :0005525 : GTP binding and also this GO :0019002 : GMP binding and also this GO :0019003 : GDP binding and also this GO :0030275 : LRR domain binding and also this GO :0032403 : protein complex binding, this GO :0005515 : protein binding, this GO :0005515 : protein binding, this GO :0005525 : GTP binding, this GO :0005525 : GTP binding, this GO :0008022 : protein C-terminus binding, this GO :0015093 : ferrous iron transmembrane transporter activity, this GO :0019002 : GMP binding, this GO :0019003 : GDP binding, this GO :0030275 : LRR domain binding, this GO :0032403 : protein complex binding, v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog, v-Ha-ras Harvey rat sarcoma viral oncogene homolog |
| Identity: |
5173 |
| Gene: |
HRAS |
More about : HRAS |
| Long gene name: |
GTPase , HRas proto-oncogene |
| Synonyms gene: |
HRAS1 |
| Synonyms gene name: |
v-Ha-ras Harvey rat sarcoma viral oncogene homolog Harvey rat sarcoma viral oncogene homolog |
| Locus: |
11p15, 5 |
| Discovery year: |
2001-06-22 |
| GenBank acession: |
AJ437024 |
| Entrez gene record: |
3265 |
| RefSeq identity: |
NM_176795 |
| Classification: |
RAS type GTPase family |
| Havana BLAST/BLAT: |
OTTHUMG00000131919 |
| Locus Specific Databases: |
LRG_506 |