| Long name: |
Cy3 Conjugated, ERK5 (Ser731+Thr733) Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
cy3 conjugation kit |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
ERK5 Ser731+Thr733 |
| Specificity: |
Recognizes Ser721+Thr723 in mice and rats, This is a highly specific antibody against ERK5 Ser731+Thr733 |
| Modification: |
Phosphorylation |
| Modification site: |
Ser731+Thr733 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
725-740/816 |
| Subcellular location: |
Nucleus, Cytoplasm |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human ERK5 around the phosphorylation sites of Ser731+Thr733 |
| Gene ID number: |
5598 |
| Swiss Prot: |
Q13164 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway, Involved in the regulation of p53/TP53 by disrupting the PML-MDM2 interaction, MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways, May be important for endothelial function and maintenance of blood vessel integrity, May have a role in muscle cell differentiation, Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression, The upstream activator of MAPK7 is the MAPK kinase MAP2K5, Upon activation, differentiation and cell survival, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C, Plays a role in various cellular processes such as proliferation |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
512, 550nm/570, 615nm |
| Synonyms: |
BMK1 ERK5 |
| Properties: |
1 of the ERK5 (Ser731+Thr733) Antibody, Conjugated can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC, Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser, This Bioss Primary Conjugated Antibodies antibody is well suited for fluorescent microscopy, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
ERK5 (Ser731+Thr733) Conjugated |
| Short name: |
Conjugated, ERK5 (Ser731+Thr733) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Cy3 |
| Alternative name: |
cyanine 3 coupled, ERK5 (Ser731+Thr733) (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
6880 |
| Gene: |
MAPK7 |
More about : MAPK7 |
| Long gene name: |
mitogen-activated protein kinase 7 |
| Synonyms gene: |
PRKM7 |
| Synonyms name: |
BMK1 kinase extracellular-signal-regulated kinase 5 |
| Locus: |
17p11, 2 |
| Discovery year: |
1998-04-28 |
| GenBank acession: |
U25278 |
| Entrez gene record: |
5598 |
| Pubmed identfication: |
10072598 7759517 |
| RefSeq identity: |
NM_139033 |
| Classification: |
Mitogen-activated protein kinases |
| Havana BLAST/BLAT: |
OTTHUMG00000059587 |