| Long name: |
FITC Conjugated, ERK1+ERK2(T185+Y187+T202+Y204) Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
ERK1+ERK2 Thr185+Tyr187+Thr202+Tyr204 |
| Specificity: |
This is a highly specific antibody against ERK1+ERK2 Thr185+Tyr187+Thr202+Tyr204 |
| Modification: |
Phosphorylation |
| Modification site: |
Thr185+Tyr187+Thr202+Tyr204 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human ERK1 around the phosphorylation site of Thr185/Tyr187+Thr202/Tyr204 |
| Gene ID number: |
5595 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Cell stimulation induces the activation of a signaling cascade, ERK1 and ERK2 are phosphorylated within the activation loop on both a Threonine and a Tyrosine residue (within a Thr-Glu-Tyr motif) by MEKs (MAPK/ERK kinases), These kinases belong to a family of serine/threonine kinases that are activated upon treatment of cells with a large variety of stimuli including mitogens, also known as MAPK (mitogen activated protein kinase) has two closely related isoforms of 44 kDa and 42 kDa, and bioactive peptides, cytokines, growth factors, hormones, respectively, the downstream effects of which have been linked to the regulation of cell growth and differentiation as well as the cytoskeleton, thereby greatly elevating the activity of ERK1&, 2, ERK (extracellular signal regulated kinase) |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
ERK1 p44mapk p44erk1 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
ERK1+ERK2(T185+Y187+T202+Y204) Conjugated |
| Short name: |
FITC Conjugated, ERK1+ERK2(T185+Y187+T202+Y204) Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, ERK1+ERK2(T185+Y187+T202+Y204) (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
6877 |
| Gene: |
MAPK3 |
More about : MAPK3 |
| Long gene name: |
mitogen-activated protein kinase 3 |
| Synonyms gene: |
PRKM3 |
| Locus: |
16p11, 2 |
| Discovery year: |
1993-11-05 |
| GenBank acession: |
M84490 |
| Entrez gene record: |
5595 |
| Pubmed identfication: |
9628824 |
| Classification: |
Mitogen-activated protein kinases |
| Havana BLAST/BLAT: |
OTTHUMG00000132149 |