| Long name: |
Biotin Conjugated, LKB1 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
LKB1 |
| Specificity: |
This is a highly specific antibody against LKB1 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
30-90/433 |
| Subcellular location: |
Membrane bound, Mitochondrion, Nucleus, Cytoplasm |
| Source: |
KLH conjugated synthetic peptide derived from human LKB1 |
| Gene ID number: |
6794 |
| Swiss Prot: |
Q15831 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Able to phosphorylate p53/TP53, Acts as a key upstream regulator of AMPK by mediating phosphorylation and activation of AMPK catalytic subunits PRKAA1 and PRKAA2 and thereby regulates processes including: inhibition of signaling pathways that promote cell growth and proliferation when energy levels are low, Acts by phosphorylating the T-loop of AMPK family proteins, Also acts as a mediator of p53/TP53-dependent apoptosis via interaction with p53/TP53: translocates to the mitochondrion during apoptosis and regulates p53/TP53-dependent apoptosis pathways, Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton, Also phosphorylates non-AMPK family proteins such as STRADA, BRSK1, BRSK2, In vein endothelial cells, Involved in DNA damage response: interacts with p53/TP53 and recruited to the CDKN1A/WAF1 promoter to participate in transcription activation, MARK1, MARK2, MARK3, MARK4, NUAK1, NUAK2, PRKAA2, PTEN and possibly p53/TP53, Regulates UV radiation-induced DNA damage response mediated by CDKN1A, Required for cortical neuron polarization by mediating phosphorylation and activation of BRSK1 and BRSK2, SIK1, SIK2, SIK3 and SNRK but not MELK, activation of autophagy when cells undergo nutrient deprivation, and B-cell differentiation in the germinal center in response to DNA damage, apoptosis and DNA damage response, cell polarity, glucose homeostasis in liver, inhibits PI3K/Akt signaling activity and thus induces apoptosis in response to the oxidant peroxynitrite (in vitro), leading to axon initiation and specification, the relevance of such result in vivo is however unclear and phosphorylation may be indirect and mediated by downstream STK11/LKB1 kinase NUAK1, thereby playing a role in various processes such as cell metabolism, thus promoting their activity: phosphorylates PRKAA1, Tumor suppressor serine/threonine-protein kinase that controls the activity of AMP-activated protein kinase (AMPK) family members |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
PJS LKB1 |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
LKB1 Conjugated |
| Short name: |
Conjugated, LKB1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, LKB1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
11389 |
| Gene: |
STK11 |
More about : STK11 |
| Long gene name: |
serine/threonine kinase 11 |
| Synonyms gene name: |
serine/threonine kinase 11 (Peutz-Jeghers syndrome) |
| Synonyms name: |
polarization-related protein LKB1 |
| Locus: |
19p13, 3 |
| Discovery year: |
1998-01-21 |
| GenBank acession: |
U63333 |
| Entrez gene record: |
6794 |
| Pubmed identfication: |
9425897 |
| RefSeq identity: |
NM_000455 |
| Havana BLAST/BLAT: |
OTTHUMG00000180118 |
| Locus Specific Databases: |
LRG_319 |