| Long name: |
FITC Conjugated, Legumain Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Legumain |
| Specificity: |
This is a highly specific antibody against Legumain |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
225-275/433 |
| Subcellular location: |
Lysosome |
| Source: |
KLH conjugated synthetic peptide derived from human Legumain |
| Gene ID number: |
5641 |
| Swiss Prot: |
Q99538 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Can also cleave aspartyl bonds slowly, May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system, Plays a role in the regulation of cell proliferation via its role in EGFR degradation (By similarity), Required for normal degradation of internalized EGFR, Required for normal lysosomal protein degradation in renal proximal tubules, especially under acidic conditions, Has a strict specificity for hydrolysis of asparaginyl bonds |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
Asparaginyl endopeptidase, LGMN, LGMN1, Legumain, PRSC1, Protease, cysteine 1, AEP |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Legumain Conjugated |
| Short name: |
FITC Conjugated, Legumain Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, Legumain (Antibody to) |
| Alternative technique: |
antibodies |