| Long name: |
Biotin Conjugated, eIF3F Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
e3F |
| Specificity: |
This is a highly specific antibody against e3F |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
135-185/357 |
| Subcellular location: |
Cytoplasm |
| Source: |
KLH conjugated synthetic peptide derived from human eIF3F |
| Gene ID number: |
8665 |
| Swiss Prot: |
O00303 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Deubiquitinates activated NOTCH1, The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation, The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC), promoting its nuclear import, thereby acting as a positive regulator of Notch signaling, which is required for several steps in the initiation of protein synthesis, Component of the eukaryotic translation initiation factor 3 (eIF-3) complex |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
Deubiquitinating enzyme eIF3f, Eukaryotic translation initiation factor 3 subunit 5, Eukaryotic translation initiation factor 3 subunit F, eIF-3-epsilon, eIF3 p47, eIF3-p47, eIF3f, EIF3S5 |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
eIF3F Conjugated |
| Short name: |
Conjugated, eIF3F Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, eIF3F (Antibody to) |
| Alternative technique: |
antibodies |