| Long name: |
ALEXA FLUOR 594 Conjugated, PKR (Thr451) Polyclonal Antibody |
| Also known as: |
Anti-PKR Thr451 PAb ALEXA FLUOR 594 |
| Category: |
Conjugated Primary Antibodies |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
PKR Thr451 |
| Specificity: |
This is a highly specific antibody against PKR Thr451 |
| Modification: |
Phosphorylation |
| Modification Site: |
Thr451 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen range: |
420-456/551 |
| Subcellular location: |
Nucleus, Cytoplasm |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human PKR around the phosphorylation site of Thr451 |
| Gene ID Number: |
5610 |
| Swiss Prot: |
P19525 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Human |
| Cross-reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the antigen: |
ATF3) involved in the expression of genes encoding proinflammatory cytokines and IFNs, Activates the NF-kappa-B pathway via interaction with IKBKB and TRAF family of proteins and activates the p38 MAP kinase pathway via interaction with MAP2K6, Also phosphorylates other substrates including p53/TP53, Can act as both a positive and negative regulator of the insulin signaling pathway (ISP), DHX9, Either as an adapter protein and/or via its kinase activity, Exerts its antiviral activity on a wide range of DNA and RNA viruses including hepatitis C virus (HCV), ILF3, IRF1, IRS1 and the HHV-1 viral protein US11, In addition to serine/threonine-protein kinase activity, Inhibits viral replication via phosphorylation of the alpha subunit of eukaryotic initiation factor 2 (EIF2S1), NF-kappa-B and insulin signaling pathways) and transcription factors (JUN, Negatively regulates ISP by inducing the inhibitory phosphorylation of insulin receptor substrate 1 (IRS1) at 'Ser-312' and positively regulates ISP via phosphorylation of PPP2R5A which activates FOXO1, PPP2R5A, STAT1, STAT3, also has tyrosine-protein kinase activity and phosphorylates CDK1 at 'Tyr-4' upon DNA damage, apoptosis, can regulate various signaling pathways (p38 MAP kinase, cell proliferation and differentiation, facilitating its ubiquitination and proteosomal degradation, hepatitis B virus (HBV), measles virus (MV) and herpes simplex virus 1 (HHV-1), this phosphorylation impairs the recycling of EIF2S1 between successive rounds of initiation leading to inhibition of translation which eventually results in shutdown of cellular and viral protein synthesis, which in turn up-regulates the expression of insulin receptor substrate 2 (IRS2), IFN-induced dsRNA-dependent serine/threonine-protein kinase which plays a key role in the innate immune response to viral infection and is also involved in the regulation of signal transduction |
| Purification: |
Purified by Protein A |
| Storage conditions: |
50% glycerol and 0, Keep refrigerated at 2 to 8 degrees Celcius for up to one year, 09% sodium azide, Store this antibody in aqueous buffered solution containing 1% BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
PKR EIF2AK1 PPP1R83 |
| Properties: |
ALEXA FLUOR they should be stored frozen at - 24°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies, C, C for long term storage and for short term at + 5°, For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
594, ALEXA FLUOR®, Alexa Fluor |
| Conjugated: |
Alexa conjugate 1 |
| Gene target: |
PKR (Thr451) |
| Short name: |
PKR (Thr451) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Label: |
ALEXA FLUOR 594 |
| Alternative name: |
ALEXA FLUOR 594, PKR (Thr451) (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
9437 |
| Gene: |
EIF2AK2 |
More about : EIF2AK2 |
| Long gene name: |
eukaryotic translation initiation factor 2 alpha kinase 2 |
| Synonyms gene: |
PRKR |
| Synonyms gene name: |
interferon-inducible double stranded RNA dependent , protein kinase |
| Synonyms name: |
protein phosphatase 1, regulatory subunit 83 |
| Locus: |
2p22, 2 |
| Discovery year: |
1992-12-04 |
| GenBank acession: |
BC057805 |
| Entrez gene record: |
5610 |
| Pubmed identfication: |
1351683 |
| RefSeq identity: |
NM_002759 |
| Classification: |
Protein phosphatase 1 regulatory subunits |
| Havana BLAST/BLAT: |
OTTHUMG00000100962 |