| Long name: |
ALEXA FLUOR 647 Conjugated, IKK alpha/beta (S176 + S180) Antibody |
| Also known as: |
Anti-IKK alpha/beta Ser176 + Ser180 PAb ALEXA FLUOR 647 |
| Category: |
Conjugated Primary Antibodies |
| Conjugated with: |
647, ALEXA FLUOR® |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
IKK alpha/beta Ser176 + Ser180 |
| Specificity: |
This is a highly specific antibody against IKK alpha/beta Ser176 + Ser180 |
| Modification: |
Phosphorylation |
| Modification Site: |
Ser176 + Ser180 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen range: |
150-200/756 |
| Subcellular location: |
Nucleus |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human IKK alpha/beta around the phosphorylation site of Ser176/Ser180 |
| Gene ID Number: |
1147 |
| Swiss Prot: |
O15111 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Cross-reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the antigen: |
Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on serine residues, Additionally, As part of the non-canonical pathway of NF-kappa-B activation, CHUK plays a key role in the negative feedback of NF-kappa-B canonical signaling to limit inflammatory gene activation, DNA damages or other cellular stresses, In turn, In turn, Modulates chromatin accessibility at NF-kappa-B-responsive promoters by phosphorylating histones H3 at 'Ser-10' that are subsequently acetylated at 'Lys-14' by CREBBP, Negatively regulates the pathway by phosphorylating the scaffold protein TAXBP1 and thus promoting the assembly of the A20/TNFAIP3 ubiquitin-editing complex (composed of A20/TNFAIP3, Participates also in the negative feedback of the non-canonical NF-kappa-B signaling pathway by phosphorylating and destabilizing MAP3K14/NIK, TAX1BP1, Therefore, These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome, Within the nucleus, and the E3 ligases ITCH and RNF11), bacterial or viral products, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes, or protection against apoptosis, phosphorylates CREBBP and consequently increases both its transcriptional and histone acetyltransferase activities, phosphorylates the CREBBP-interacting protein NCOA3, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, these complexes regulate genes encoding molecules involved in B-cell survival and lymphoid organogenesis, Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines |
| Purification: |
Purified by Protein A |
| Storage conditions: |
50% glycerol and 0, Keep refrigerated at 2 to 8 degrees Celcius for up to one year, 09% sodium azide, Store this antibody in aqueous buffered solution containing 1% BSA |
| Excitation emission: |
650nm/665nm |
| Synonyms: |
CHUK, Conserved helix-loop-helix ubiquitous kinase, I-kappa-B kinase 1, I-kappa-B kinase alpha, IKBKA, IKK-A, IKK-alpha, IKKA, IkappaB kinase, Inhibitor of nuclear factor kappa-B kinase subunit alpha, NFKBIKA, Nuclear factor NF-kappa-B inhibitor kinase alpha, TCF-16, TCF16, Transcription factor 16, IKK1 |
| Properties: |
ALEXA FLUOR made this Alexa Fluor 633 conjugate that can be used in multi-color flow cytometry with instruments equipped with a second red laser or red diode, ALEXA FLUOR they should be stored frozen at - 24°, Alexa Fluor 633 is a practical alternative to APC as well as Cy5, Bioss Primary Conjugated Antibodies, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies, It is detected in the FL4 detector of the core's upgraded 2-laser FACScans, Like other Alexa Fluor dyes, exhibits uncommon photo stability, making it an ideal choice for fluorescent microscopy, the IKK alpha/beta (S176 + S180) Antibody, C, C for long term storage and for short term at + 5°, For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
ALEXA FLUOR 647, Alexa Fluor |
| Gene target: |
IKK alpha/beta (S176 + S180) |
| Short name: |
IKK alpha/beta (S176 + S180) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Label: |
ALEXA FLUOR 647 |
| Alternative name: |
ALEXA FLUOR 647, IKK a/b (S176 and S180) (Antibody to) |
| Alternative technique: |
antibodies |