| Long name: |
FITC Conjugated, HER2(Tyr1221 + Tyr1222) Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
HER2 Tyr1221 + Tyr1222 |
| Specificity: |
This is a highly specific antibody against HER2 Tyr1221 + Tyr1222 |
| Modification: |
Phosphorylation |
| Modification site: |
Tyr1221 + Tyr1222 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human HER2 around the phosphorylation site of Tyr1221/1222 |
| Gene ID number: |
2064 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, Alternative splicing results in several additional transcript variants, Amplification and/or overexpression of this gene has been reported in numerous cancers, However, Ile654/Ile655, Jul 2008], This protein has no ligand binding domain of its own and therefore cannot bind growth factors, [provided by RefSeq, including breast and ovarian tumors, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, shown here, some encoding different isoforms and others that have not been fully characterized, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase, with the most common allele, This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
NEU HER-2 CD340 HER2 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
HER2(Tyr1221 + Tyr1222) Conjugated |
| Short name: |
FITC Conjugated, HER2(Tyr1221 + Tyr1222) Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, HER2(Tyr1221 and Tyr1222) (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
3430 |
| Gene: |
ERBB2 |
More about : ERBB2 |
| Long gene name: |
erb-b2 receptor tyrosine kinase 2 |
| Synonyms gene: |
NGL |
| Synonyms gene name: |
v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog) v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 |
| Synonyms name: |
neuro/glioblastoma derived oncogene homolog human epidermal growth factor receptor 2 |
| Locus: |
17q12 |
| Discovery year: |
2001-06-22 |
| GenBank acession: |
X03363 |
| Entrez gene record: |
2064 |
| RefSeq identity: |
NM_004448 |
| Classification: |
CD molecules Minor histocompatibility antigens Erb-b2 receptor tyrosine kinases |
| Havana BLAST/BLAT: |
OTTHUMG00000179300 |
| Locus Specific Databases: |
LRG_724 |