| Long name: |
Biotin Conjugated, Histone H2A, X (Ser140) Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Histone H2A, X Ser140 |
| Specificity: |
This phosphorylation site is homologous to that of Ser140 in Mouse and Rat, This is a highly specific antibody against Histone H2A, X Ser140 |
| Modification: |
Phosphorylation |
| Modification site: |
Ser140 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
100-142/143 |
| Subcellular location: |
Nucleus |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human Histone H2AX around the phosphorylation site of Ser140 |
| Gene ID number: |
3014 |
| Swiss Prot: |
P16104 |
| Tested applications: |
IHC-P |
| Recommended dilutions: |
IHC-P(1:100-500) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
DNA accessibility is regulated via a complex set of post-translational modifications of histones, DNA repair, DNA replication and chromosomal stability, Histones thereby play a central role in transcription regulation, Nucleosomes wrap and compact DNA into chromatin, Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation, also called histone code, and nucleosome remodeling, limiting DNA accessibility to the cellular machineries which require DNA as a template, Variant histone H2A which replaces conventional H2A in a subset of nucleosomes |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
H2A, H2A/X, H2AFX, Histone H2A, Histone H2AX, H2AX, X, X |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Histone H2A, X (Ser140) Conjugated |
| Short name: |
Conjugated, Histone H2A, X (Ser140) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, Histone H2A, X (Ser140) (Antibody to) |
| Alternative technique: |
antibodies |