| Long name: |
Biotin Conjugated, A Raf (Ser214) Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
A Raf Ser214 |
| Specificity: |
This is a highly specific antibody against A Raf Ser214 |
| Modification: |
Phosphorylation |
| Modification site: |
Ser214 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human A Raf around the phosphorylation site of Ser214 |
| Gene ID number: |
369 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
14-3-3 is also known to be an activator of RAFs, A RAF is expressed at a very high concentration in urogenital and kidney tissues, Activated RAF directly phosphorylates and activates MEK, RAF 1 and B RAF, RAF 1 is known to be downstream of Ras and binds to it by an N terminal noncatalytic domain, These proteins are instrumental in forwarding the stimulus generated by mitogens from monomeric G proteins to the MAP kinase pathway, This domain is conserved in A RAF and B RAF, The RAF family of signaling proteins has three isoforms: A RAF |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
A Raf proto oncogene serine/threonine protein kinase, ARAF, ARAF 1, ARAF1, ARAF_HUMAN, ARaf proto oncogene serine/threonine protein kinase, Oncogene Araf1, Oncogene PKS2, PKS, PKS 2, PKS2, Proto oncogene Pks, RAFA 1, RAFA1, Ras binding protein DA Raf, v raf murine sarcoma 3611 viral oncogene homolog, v raf murine sarcoma 3611 viral oncogene homolog 1, v raf oncogene homolog 1 murine sarcoma 3611 virus, A raf 1 |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
A Raf (Ser214) Conjugated |
| Short name: |
Conjugated, A Raf (Ser214) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, A Raf (Ser214) (Antibody to) |
| Alternative technique: |
antibodies |