| Long name: |
FITC Conjugated, Smad2 (Ser465) Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Smad2 Ser465 |
| Specificity: |
This is a highly specific antibody against Smad2 Ser465 |
| Modification: |
Phosphorylation |
| Modification site: |
Ser465 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human Smad2 around Ser465 |
| Gene ID number: |
4087 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
It belongs to the SMAD family of proteins, It interacts with SMAD anchor for receptor activation (SARA), It is subsequently transferred to the nucleus where it forms complexes with other proteins and acts as a transcription factor, Like many other TGF-Beta family members SMAD2 is involved in cell signalling, Mothers against DPP homolog 2, SMAD 2, SMAD2 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase, SMAD2 modulates signals of activin and TGF-Beta's, Smad2 (Mothers against decapentaplegic homolog 2, The binding of ligands causes the phosphorylation of the SMAD2 protein and the dissociation from SARA and the association with SMAD4, as its name describes, is a homolog of the Drosophila gene: ", which belong to the TGF-Beta superfamily of modulators, ), Mothers against decepentaplegic", SMAD2 or Mothers against decapentaplegic homolog 2 is a polypeptide that |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
JV18, JV18 1, JV181, MAD, MAD Related Protein 2, MADH2, MADR2, MGC22139, MGC34440, Mothers Against Decapentaplegic Homolog 2, Mothers against DPP homolog 2, Mothers against decapentaplegic homolog 2, SMAD, SMAD 2, SMAD 2, SMAD2, SMAD2_HUMAN, Smad2, Smad2S465, hMAD 2, hSMAD2, mothers against DPP homolog 2, p-Smad2Ser465, Smad2Ser465 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Smad2 (Ser465) Conjugated |
| Short name: |
FITC Conjugated, Smad2 (Ser465) Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, SMAD family member 2 (Ser465) (Antibody to) |
| Alternative technique: |
antibodies |